<原著>モルモット鼓膜穿孔の治癒過程における細胞成長因子の影響
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Effects of EGF (epidermal growth factor) and TGF-β (transforming growth factor-β) on the healing of tympanic membrane perforations were studied in guinea pigs. Under pentobarbital sodium anaesthesia, tympanic perforations of the same size and shape were made in the posterosuperior quadrants of the pars tensa by means of an electrical burn through the external auditory meatus. The animals were divided into four groups : Group 1 which was treated daily and topically with EGF and insulin, Group 2 which was also treated daily and topically with TGF-β and insulin, Group 3 which was treated daily and topically only with insulin, and Group 4 which was left to heal spontaneously. The tympanic membranes of all animals were observed daily under ether anaesthesia by means of operating microscopy and every second day through a light microscope. All perforations were of a uniform size with a vertical diameter parallel to the longitudinal axis of the handle of the malleus being 1.54±0.16mm, and its horizontal diameter parallel to the transverse axis of the handle of the malleus being 1.36±0.21mm. The perforations closed in 8.0±1.2 days in ears treated with EGF, in 9.8±1.7 days in insulintreated ears, and in 10.4±1.5 days in control subjects. The time taken for the perforations of Group 1 subjects to close was statistically different from that of the other groups. Considerable delay was observed in the closing of Group 2 perforations. According to histological investigation, the maximum thickness of the outer layer and that of the middle layer were measured at the margin of the perforation. In Group 1,epithelial proliferation occurred more rapidly than in the control group, and the middle layer of the tympanic membrane appeared to be less thickened than in the control group. In Group 2,the epithelial proliferation was not significantly different from that of the control group, but the middle layer appeared to be much thinner than in the control. The epithelial layer seems to play an important role in the healing process of the tympanic membrane.
- 近畿大学の論文
- 1989-09-25
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