SHR-SPの脳実質中のplasminogen activatorについて(I 発生病理)(A 基礎的研究)

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The present study was undertaken to purify plasminogen activator (PA) from the brain tissue of stroke-prone spontaneously hypertensive rats (SHR-SP) and Wistar-Kyoto rats (WKY), and to compare PA content as well as biological properties of PA between SHR-SP and WKY, since the mechanism of stroke remains still unclear. Brain tissue of six-month-old male rats was homogenized in 0.15M KCl solution, and 0.15M KCl soluble fraction as well as 0.15M KCl insoluble fraction was used for the purification of PA. PA was purified by the methods of ammonium sulfate precipitation, zinc chelate-Sepharose chromatography, and concanavalin A-Sepharose chromatography. Purified PA had high specific activity (>98 IU/A_<280mm>) as compared with crude homogenates (<0.2 IU/A_<280mm>), with the purification factor of about 1,000-3,000. The molecular weight of PA was observed by electrophoretic enzymography, showing enzymatically active and single band with the molecular weight of 72k in both SHR and WKY. Purified PA from both SHR and WKY had affinity to fibrin-Sepharose, but the degree was not so high as the tissue-type plasminogen activator (t-PA) purified from the culture medium of melanoma (Bowes). Total content of PA in the brain tissue of SHR-SP was estimated at about twice as much as WKY. The results suggest quantitative difference of PA in the brain tissue may exist between pathogenic (SHR-SP) and normal (WKY) states, but qualitative difference in PA was not observed.
近畿大学の論文
1985-12-30

SHR-SPの脳実質中のplasminogen activatorについて(I 発生病理)(A 基礎的研究)

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