<ORIGINAL REPORT>FLUOROMETRIC MEASUREMENT OF 3-METHYLCHOLANTHRENE AND ITS METABOLITES IN TISSUE. I. MEASUREMENT OF 3-METHYLCHOLANTHRENE IN ORAGANIC SOLVENTS
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概要
- 論文の詳細を見る
この論文は国立情報学研究所の学術雑誌公開支援事業により電子化されました。1. Fluorometry of 3-MC was carried out with a Shimazu Fluorospectrophotometer Model GF-16 with a sensitivity for this hydrocarbon of about 1ng. 2. Fluorometry of the hydrocarbon in benzene should be done at concentrations less than 1μg per ml by excitation at 300nm wavelength and fluorescence measurement at 400nm. 3. To avoid degradation by UV light illumination, the measurement should be performed as quickly as possible. 4. Lanolin, as well as sesame and corn oils, has its own fluorescence and this results in a significant decrease of 3-MC fluorescence. However, the quenching can be avoided by simple dilution with benzene of the solution containing these substances. 5. 3-MC is stable in Freund's incomplete adjuvant for at least 6 weeks.
- 京都大学の論文
- 1974-12-30
著者
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Takahashi Gonya
Department Of Pathology Chest Disease Research Institute Kyoto University
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YASUHIRA Kimino
Department of Pathology, Chest Disease Research Institute, Kyoto University
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Yasuhira Kimino
Department Of Pathology Chest Disease Research Institute Kyoto University
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安平 公夫
Department of Pathology, Chest Disease Research Institute, Kyoto University
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- FLUOROMETRIC MEASUREMENT OF 3-METHYLCHOLANTHRENE AND ITS METABOLITES IN TISSUE. I. MEASUREMENT OF 3-METHYLCHOLANTHRENE IN ORAGANIC SOLVENTS