Endogenous Alkaline Phosphatase Activities in Rat Tissues

概要

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Endogenous ALP activities were examined in various tissues using different substrates, Nitroblue tetrazolium chloride/ 5-brom-4-chloro-3-indolyl phosphate (NBT/BCIP), Vector Blue, and Vector Red. Comparative analysis demonstrated marked differences of signal intensities depending on the substrates. Catalyzed NBT/BCIP Products deposited heavily and proved to be sensitive for detecting signals, the Vector Blue the second, and the Vector Red less sensitive, although the three substrates yielded their own color precipitations. Endogenous ALP activities were observed in all tissues examined. The other factors such as histological procedures also affected endogenous ALP activities. Frozen tissues either fixed or unfixed exhibited well-preserved ALP activities, while the signals in paraffin-embedded materials were eliminated from most tissues, except for small intestine. An ALP inhibitor, or Levamisole, actually suppressed the enzyme activities in various tissues but not always successful nor perfect. The present study provokes cautious information necessary to control endogenous ALP activities in target tissues of interest when exogenous ALP is applied as a tool of signal detection.