<SESSION>CHROMOSOMAL DAMAGE IN HUMAN SPERMATOZOA CAUSED BY IN VITRO IRRADIATION OF TRITIUM β-RAYS

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Effects of tritium (HTO) β-rays on human sperm chromosomes were studied using our interspecific in vitro fertilization system between human spermatozoa and zona-free hamster oocytes. Semen samples were treated with media containing 1.53 - 24.30 mCi/m1 HTO for about 80 minutes. Six hundred and forty-six spermatozoa were karyotyped in the controls and 1,383 spermatozoa in the irradiated groups. The incidence of spermatozoa with structural chromosome aberrations increased linearly with increasing dosage. Breakage type aberrations occurred far more frequently than the exchange type. Chromosome type aberrations appeared far more frequently than the chromatid type. The RBE values of HTO β-rays relative to X-rays were calculated for the above-mentioned 5 indices, respectively. Their RBE values ranged from 1.91 to 3.18 when the absorbed dose was estimated to be the minimum, whereas the values ranged between 1.05 and 1.75 when the absorbed dose was estimated to be the maximum.
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