A Human Leukemia Cell Line CCRF-HSB-2 Is Useful for the Screening of Antitumor Activity

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p53 is one of the critical factors for the activity of antitumor agents, and chemicals that induce p53 are potential new antitumor agents. Four human leukemia cell lines (BALL-1, CCRF-HSB-2, KU812, MOLT-4) were treated with some DNA damaging stresses, resulting in accumulation of p53 and induction of apoptosis in CCRF-HSB-2, which has wild type p53. Induction of p21 and G1 arrest was also detected after the DNA damage to this cell line. Gamma irradiations, cisplatin and etoposide were used as DNA damaging stresses. Induction of p53 was detected by Western blot analysis, and apoptosis was detected by DNA fragmentation analysis on agarose gel. The cell cycle was analyzed by flow cytometry. Apoptosis was induced in association with accumulation of p53 after 8 h of DNA damaging treatments. However,the cell lines which contained only mutant p53 gene failed to accumulate p53 or p21, and lacked apoptosis and G1 arrest. The expression of bax after gamma irradiation in the same four human leukemia cell lines (BALL-1, CCRF-HSB-2, KU812, MOLT-4) was also examined. This result suggests that an unidentified gene(s) which was induced by p53 may trigger the apoptosis in CCRF-HSB-2 cells and that induction of wild-type p53 produces the apoptosis in this cell line. Our study showed that CCRF-HSB-2 has the potential to assist in the development of new antitumor agents
札幌医科大学の論文