Immortalization of Primary Rat Cells by Reciprocal Complementations of Human Papillomavirus Type 16 E7 and Adenovirus Type 5 E1A Mutants
スポンサーリンク
概要
- 論文の詳細を見る
Primary baby rat kidney (BRK) cells were transfected with two different non-immortalizing mutants of adenovirus type 5 (Ad5) E1A(s) and/or human papillomavirus type 16 (HPV16) E7(s) to test whether immortalization was induced by the genetic complementation. Co-transfection of an RB-nonbinding E1A mutant (d1922/947) and N-terminal E7 mutant (2PRO) formed transformed foci on BRK cells, but a combination of an N-terminal E1A mutant (NTdl598) and RB-nonbinding E7 mutant (24GLY) did not. However, NTd1598 plus 24GLY as well as dl922/947 plus 2PRO produced immortalized BRK cell lines. The cell lines immortalized by dl922/947 plus 2PRO showed a morphology similar to those immortalized by Ad5 E1A ; while cell lines immortalized by NTdl598 plus 24GLY showed a morphology similar to those immortalized by HPV16 E7. These results suggest that the RB-binding regions of Ad5 E1A and HPV16 E7 are mutually replaceable, and that the N-terminal function of E1A is essential for focus formation and maintenance of transformation morphology. A combination of RB-nonbinding mutants, E1A dl922/947 plus E7 24GLY, was able to immortalize BRK cells when introduced together with the Ad5 E1B 19K gene. The Ad5 E1B 55K and Bcl-2, which also possess anti-apoptotic activity, did not increase colony formation of and/or immortalize BRK cells when co-transfected with dl922/947 plus 24GLY. In a CAT assay E1B 19K, but not Bc1-2, enhanced E2F-dependent CAT transactivation by Ad5 ElA or dl922/947. Thus, it is suggested that E1B 19K might complement RB-nonbinding mutants by means of two activities, suppression of apoptosis and enhancement of E2F-dependent transactivation.
- 札幌医科大学の論文
著者
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YOSHIDA Koichi
Department of Dental Materials Science, School of Life Dentistry at Tokyo, The Nippon Dental Univers
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YAMASHITA TOSHIHIKO
Department of Orthopaedic Surgery, Sapporo Medical University School of Medicine
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YAMASHITA Toshiharu
Department of Dermatology, Cancer Research Institute, Sapporo Medical University
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Yamashita Toshihiko
Department Of Orthopaedic Surgery Sapporo Medical University School Of Medicine
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Yamashita T
Department Of Orthopedic Surgery Sapporo Medical University School Of Medicine
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Yamashita Toshiharu
Department Of Dermatology Cancer Research Institute Sapporo Medical University
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Kagiichi Kimura
Department Of Molecular Biology Cancer Research Institute And Second Department Of Pathology Sapporo
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Tomiyoshi Yasuo
Kitago Orthopedic Clinic
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Yamashita T
Department Of Orthopaedic Surgery Sapporo Medical University School Of Medicine
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Yoshida Koichi
Department Of Dental Materials Science School Of Life Dentistry At Tokyo The Nippon Dental Universit
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Yoshida Koichi
Department Of Molecular Biology Cancer Research Institute Sapporo Medical University School Of Medic
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Yamashita Toshihiko
Department Of Orthopedic Sugery Sapporo Medical University School Of Medicine
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Kimura Kagiichi
Department of Molecular biology, Cancer Research Institute, and Second Department of Pathology, Sapporo Medical University School of medicine
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