Immortalization of Primary Rat Cells by Reciprocal Complementations of Human Papillomavirus Type 16 E7 and Adenovirus Type 5 E1A Mutants
スポンサーリンク
概要
- 論文の詳細を見る
Primary baby rat kidney (BRK) cells were transfected with two different non-immortalizing mutants of adenovirus type 5 (Ad5) E1A(s) and/or human papillomavirus type 16 (HPV16) E7(s) to test whether immortalization was induced by the genetic complementation. Co-transfection of an RB-nonbinding E1A mutant (d1922/947) and N-terminal E7 mutant (2PRO) formed transformed foci on BRK cells, but a combination of an N-terminal E1A mutant (NTdl598) and RB-nonbinding E7 mutant (24GLY) did not. However, NTd1598 plus 24GLY as well as dl922/947 plus 2PRO produced immortalized BRK cell lines. The cell lines immortalized by dl922/947 plus 2PRO showed a morphology similar to those immortalized by Ad5 E1A ; while cell lines immortalized by NTdl598 plus 24GLY showed a morphology similar to those immortalized by HPV16 E7. These results suggest that the RB-binding regions of Ad5 E1A and HPV16 E7 are mutually replaceable, and that the N-terminal function of E1A is essential for focus formation and maintenance of transformation morphology. A combination of RB-nonbinding mutants, E1A dl922/947 plus E7 24GLY, was able to immortalize BRK cells when introduced together with the Ad5 E1B 19K gene. The Ad5 E1B 55K and Bcl-2, which also possess anti-apoptotic activity, did not increase colony formation of and/or immortalize BRK cells when co-transfected with dl922/947 plus 24GLY. In a CAT assay E1B 19K, but not Bc1-2, enhanced E2F-dependent CAT transactivation by Ad5 ElA or dl922/947. Thus, it is suggested that E1B 19K might complement RB-nonbinding mutants by means of two activities, suppression of apoptosis and enhancement of E2F-dependent transactivation.
- 札幌医科大学の論文
著者
-
YOSHIDA Koichi
Department of Dental Materials Science, School of Life Dentistry at Tokyo, The Nippon Dental Univers
-
YAMASHITA TOSHIHIKO
Department of Orthopaedic Surgery, Sapporo Medical University School of Medicine
-
YAMASHITA Toshiharu
Department of Dermatology, Cancer Research Institute, Sapporo Medical University
-
Yamashita Toshihiko
Department Of Orthopaedic Surgery Sapporo Medical University School Of Medicine
-
Yamashita T
Department Of Orthopedic Surgery Sapporo Medical University School Of Medicine
-
Yamashita Toshiharu
Department Of Dermatology Cancer Research Institute Sapporo Medical University
-
Kagiichi Kimura
Department Of Molecular Biology Cancer Research Institute And Second Department Of Pathology Sapporo
-
Tomiyoshi Yasuo
Kitago Orthopedic Clinic
-
Yamashita T
Department Of Orthopaedic Surgery Sapporo Medical University School Of Medicine
-
Yoshida Koichi
Department Of Dental Materials Science School Of Life Dentistry At Tokyo The Nippon Dental Universit
-
Yoshida Koichi
Department Of Molecular Biology Cancer Research Institute Sapporo Medical University School Of Medic
-
Yamashita Toshihiko
Department Of Orthopedic Sugery Sapporo Medical University School Of Medicine
-
Kimura Kagiichi
Department of Molecular biology, Cancer Research Institute, and Second Department of Pathology, Sapporo Medical University School of medicine
関連論文
- Color change capacity of dental resin mixed with silver methacrylate caused by light irradiation and heating
- Periosteal chondrosarcoma with microscopic cortical invasion
- Cancer epigenomics : Implications of DNA methylation in personalized cancer therapy
- Gene Expression Screening Using a cDNA Macroarray to Clarify the Mechanisms of Peritoneal Dissemination of Pancreatic Cancer
- A Novel Nude Mouse Model of Liver Metastasis and Peritoneal Dissemination From the Same Human Pancreatic Cancer Line
- A Novel Experimental Mouse Model of Peritoneal Dissemination of Human Gastric Cancer Cells: Analysis of the Mechanism of Peritoneal Dissemination Using cDNA Macroarrays
- A New Peritoneal Dissemination Model Established from the Human Pancreatic Cancer Cell Line
- Induction of Cytotoxic T Lymphocytes from Peripheral Blood of Human Histocompatibility Antigen (HLA)-A31^+ Gastric Cancer Patients by in vitro Stimulation with Antigenic Peptide of Signet Ring Cell Carcinoma
- NATURAL ANTIGENIC PEPTIDES OF GASTRIC SIGNET RING CELL CARCINOMAS
- Scythe/BAT3 regulates apoptotic cell death induced by papillomavirus binding factor in human osteosarcoma
- Prognostic impact and immunogenicity of a novel osteosarcoma antigen, papillomavirus binding factor, in patients with osteosarcoma
- Development of an Ulcer in the Side-to-side Anastomosis of a Jejunal Pouch After Proximal Gastrectomy Reconstructed by Jejunal Interposition : Report of a Case
- Separate Functions of α_2β_1 and α_3β_1 Integrins in the Metastatic Process of Human Gastric Carcinoma
- Giant Peritoneal Loose Body in the Pelvic Cavity : Report of a Case
- Transanal Repair of Rectourethral Fistula After a Radical Retropubic Prostatectomy : Report of a Case
- Septic Thrombophlebitis of the Portal and Superior Mesenteric Veins as a Complication of Appendicitis : Report of a Case
- Adenovirus-mediated Transfer of p53-related Genes Induces Apoptosis of Human Cancer Cells
- Functional outcomes after arthroscopic treatment of lateral epicondylitis
- Changes in urinary NTX levels in patients with primary osteoporosis undergoing long-term bisphosphonate treatment
- A significant improvement in lower limb pain after treatment with alendronate in two cases of Camurati-Engelmann disease
- Prevention of postoperative progression of pulmonary metastases in osteosarcoma by antiangiogenic therapy using endostatin
- Ovariectomy fails to augment bone resorption and marrow B lymphopoiesis in granulocyte colony-stimulating factor transgenic mice
- Predictive factors for vascularized iliac bone graft for nontraumatic osteonecrosis of the femoral head
- Multiple insufficiency fractures with severe osteoporosis
- Laminar configuration of the transverse carpal ligament
- 4-S-Cysteaminylphenol-loaded magnetite cationic liposomes for combination therapy of hyperthermia with chemotherapy against malignant melanoma
- Prognostic significance of HLA class I expression in osteosarcoma defined by anti-pan HLA class I monoclonal antibody, EMR8-5
- Chemokine Profile of Herniated Intervertebral Discs Infiltrated With Monocytes and Macrophages
- Complications associated with bone cementing for the treatment of giant cell tumors of bone
- Traumatic Paraplegia in Snowboarders
- Immunophenotypic Analysis of the Inflammatory Infiltrates in Herniated Intervertebral Discs
- Insufficiency fracture of the spine : a prospective analysis based on radiographic and scintigraphic diagnosis
- Giant cell tumor of the sacrum treated with selective arterial embolization
- Recognition by cellular and humoral autologous immunity in a human osteosarcoma cell line
- Over-Expression of the Testis-Specific Gene TSGA10 in Cancers and Its Immunogenicity
- A Case of Small Pancreatic Ductal Carcinoma Accompanied by Intraductal Mucin-Producing Pancreatic Tumors
- Clinical Survey of the Results on Colorectal Surgery in the Elderly
- Highly Conserved Antigenic Structure of the Factor VIII C2 Domain in Some Mammals
- Adenovirus Type 5 E1A Immortalizes Primary Rat Cells Expressing Wild-Type p53
- Primary total hip arthroplasty with Asian-type AML total hip prosthesis : follow-up for more than 10 years
- Morphological changes of the dorsal root ganglion in a patient with herpes zoster seen by magnetic resonance imaging
- Scoliosis in cat cry syndrome
- A Quantitative Analysis of Sensory Function in Lumbar Radiculopathy Using Current Perception Threshold Testing
- Clear Cell Meningioma of Cauda Equina in a 10-year-old Child 〈Case Report〉
- Scoliosis After Treatment for Neuroblastoma 〈Case Report〉
- Underutilization of antiosteoporotic drugs by orthopedic surgeons for prevention of a secondary osteoporotic fracture
- Session 1 : Telehealth-Communication 1. Prevention and Treatment of Hip Fracture Caused by Osteoporosis
- Coexistence of Chiari 2 malformation and moyamoya syndrome in a 17-year-old girl.
- Wild-type p53 Expression Overcomes p21-mediated G1 Arrest and Induces Apoptosis in Cancer Cells Expressing Bax
- A Case of Systemic Osteomyelitides Due to Mycobacterium avium
- Combined administration of basic fibroblast growth factor protein and the hepatocyte growth factor gene enhances the regeneration of dermis in acute incisional wounds
- Detection of tyrosinase and tyrosinase-related protein 1 sequences from peripheral blood of melanoma patients using reverse transcription-polymerase chain reaction
- Does the fabella contribute to the reinforcement of the posterolateral corner of the knee by inducing the development of associated ligaments?
- The serum level of bone-specific alkaline phosphatase activity is associated with aortic calcification in osteoporosis patients
- Fatigue subcapital fracture of the femur after the removal of the hip plate in transtrochanteric rotational osteotomy
- Inhibition of Osteopontin Reduces Liver Metastasis of Human Pancreatic Cancer Xenografts Injected into the Spleen in a Mouse Model
- Phase-independent Inhibition by Retinoic Acid of Mineralization Correlated with Loss of Tetranectin Expression in a Human Osteoblastic Cell Line
- Molecular Analysis of Ewing's Sarcoma: Another Fusion Gene, EWS-E1AF, Available for Diagnosis
- PROPRIOCEPTORS : PHYSIOLOGIC and MORPHOLOGIC CHARACTERISTICS
- Measurement of subclavicular pressure on the subclavian artery and brachial plexus in the costoclavicular space during provocative positioning for thoracic outlet syndrome
- Fracture of the distal end of the fibula through a persistent physis in an adult with fracture of the medial malleolus
- Melanoma-targeted chemo-thermo-immuno (CTI)-therapy using N-propionyl-4-S-cysteaminylphenol-magnetite nanoparticles elicits CTL response via heat shock protein-peptide complex release
- Effects of raloxifene treatment on the structural geometry of the proximal femur in Japanese women with osteoporosis
- Developmental Mechanism of Angina Pectoris and Action of Coronary Vasodilators
- Exercise Test and Anoxia Test in the Diagnosis of Angina Pectoris : A Comparative Study
- Mechanosensitive Afferent Units in the Lumbar Posterior Longitudinal Ligament
- Immortalization of Primary Rat Cells by Reciprocal Complementations of Human Papillomavirus Type 16 E7 and Adenovirus Type 5 E1A Mutants
- Topographical histology of the posterolateral corner of the knee, with special reference to laminar configurations around the popliteus tendon : a study of elderly Japanese and late-stage fetuses
- Two Transcription Factors, E1AF and N-myc, Correlate with the Invasiveness of Neuroblastoma Cell Lines
- Morphometry of the lumbar zygapophyseal facet capsule and cartilage with special reference to degenerative osteoarthritic changes : an anatomical study using fresh cadavers of elderly Japanese and Korean subjects
- Lateral ligament injury of the ankle and associated articular cartilage degeneration in the talocrural joint : anatomic study using elderly cadavers
- Design of a pragmatic approach to evaluate the effectiveness of concurrent treatment for the prevention of osteoporotic fractures
- Phase I clinical trial of survivin-derived peptide vaccine therapy for patients with advanced or recurrent oral cancer
- Hip structure analysis of bisphosphonate-treated Japanese postmenopausal women with osteoporosis
- 23. In Transsphenoidal Approach to Pituitary Tumors in our Department
- Bone and/or joint attachment is a risk factor for local recurrence of myxofibrosarcoma
- Course of NTX changes under continuous bisphosphonate treatment in cases of NTX over-reduction due to long-term treatment with bisphosphonates
- Activation of glial cells in the spinal cord of a model of lumbar radiculopathy
- Autologous CTL response against cancer stem-like cells/cancer-initiating cells of bone malignant fibrous histiocytoma
- Early experience using the da Vinci Surgical System for the treatment of mediastinal tumors
- Coexistence of Chiari 2 Malformation and Moyamoya Syndrome in a 17-Year-Old Girl : Case Report
- CAMAC Data Taking System for Experiments Using Continuous Ion Beams from a Cyclotron
- De novo follicular regeneration of the skin by wingless int 3 and bone morphogenetic protein 2 genes introduced into dermal fibroblasts and fibroblast growth factor-2 protein
- Applicability of radiocolloids, blue dyes and fluorescent indocyanine green to sentinel node biopsy in melanoma
- Adenine-and-Uridine-rich element RNA-binding factor 1 (AUF1) as an additional marker in human gliomas