<Original>Improvement on Preparation of RNA from Differentiating Xylem Tissue of Eucalyptus camaldulensis L.

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概要

• 論文の詳細を見る

• この論文は国立情報学研究所の学術雑誌公開支援事業により電子化されました。The CTAB method for RNA isolation in differentiating xylem has improved in the treatment of tissues with methanol containing DTT and the addition of vanadyl ribonucleosides in to the extraction buffer. Vanadyl ribonucleosides were supposed to inhibit the binding of polyphenols to RNA also, not only inhibited RNase activity. The yield of RNA was more than 270 μg/g of tissue in average, and poly(A)^+ RNA was subsequently purified by using an oligo-dT cellulose column with about 0.7% recovery of total RNA.

• 京都大学の論文
• 1997-09-30

著者

• Ito Kazuya

  Kameyama Research Center Forestry Research Institute

• ITO Kazuya

  Forestry Res.Inst., Oji Paper Co.Ltd

• Baba Kei'ichi

  Laboratory Of Cell Structure And Function Wood Research Institute Kyoto University

• Baba Kei′ich

  Laboratory of Cell Structure and Function, WOOD RESEARCH INSTITUTE, KYOTO UNIVERSITY

関連論文

• CLONING OF cDNA INVOLVING TENSION WOODFORMATION
• Effect of alkali extraction on the lignin monomeric composition in Eucalyptus
• Relations between various extracted basic densities and wood chemical components in Eucalyptus globulus
• Nucleotide Sequences and Homology Search of cDNAs expressed on the Early Stage of Tension Wood Formation
• CHANGES OF GENE EXPRESSION INVOLVING TENSION WOOD FORMATION
• Improvement on Preparation of RNA from Differentiating Xylem Tissue of Eucalyptus camaldulensis L.
• Expression of a Ruminococcus albus Cellulase Gene in Tobacco Suspension Cells

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