<Originals>Behavioral and morphological alterations in rats prenatally treated with methylazoxymethanol acetate (MAM)

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概要

• 論文の詳細を見る

• Methylazoxymethanol acetate (MAM) is an aglycon of cycasine contained in the cycad seed, and cytotoxic against neuroblasts. In order to investigate brain function impairments (especially hyperactivity), MAM was administered once intraperitoneally at 30mg/kg to female Crj-CD rats on Days 12,13,15,and 18 of gestation (Day 0=day of observation of vaginal plug). In the pups exposed to MAM on Day 13 of gestation, reflexes and motor coordination before weaning, and the activity level and learning ability after weaning were studied. In the pups exposed to MAM on Days 12,15,and 18 of gestation, activity level before weaning were analyzed, and brain tissue damage was studied 8 weeks after birth. The effects of MAM exposure on both the absolute and relative brain weights were greatest in those exposed on Day 12. Changes in skeletal development in the pups exposed to MAM on Day 13 of gestation were limited to the cranial region. Morphological abnormalities of the presphenoid bone in the cranial base were observed, suggesting impaired development extending to the central optic tract. Temporary delays of the reflex development of the pups exposed to MAM on Day 13 of gestation were noted. Similarly, delays in the development of limb motor coordination were observed in males 18-21 days after birth in a swimming test. The activity level in an open-field test was significantly higher in the animals exposed to MAM on Days 12,13,and 15 of gestation as compared with the controls. The increase in the activity level at weaning was the most notable. Learning (memory) on single T-maze and multiple T-maze tasks in the animals exposed to MAM on Day 13 of gestation was slower than in the control group. Various brain tissue abnormalities such as heterotopic aggregation of neurons and disturbance of fiber arrangement were noted in the cerebral cortex and the hippocampus of the animals exposed to MAM on Day 15 of gestation. These findings suggest that animal models of various impairments of brain development including congenital micrencephaly syndrome and hyperactive pups can be produced by appropriate selection of the time of exposure to MAM.

• 近畿大学の論文

著者

• Yamamoto Y

  Kinki Univ. School Of Medicine Osaka Jpn

• Yamamoto Yoshitame

  First Department Of Anatomy Kinki University School Of Medicine

関連論文

• Early postnatal development and learning ability of the rat offspring prenatally exposed to methylazoxymethanol acetate
• Behavioral and morphological alterations in rats prenatally treated with methylazoxymethanol acetate (MAM)

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