<Originals>Two distinct nuclear factors, Sp1 and RBF-1,maintaining constitutive promoter activity of human angiotensinogen gene

スポンサーリンク

概要

• 論文の詳細を見る

• Angiotensinogen, the protein precursor of angiotensin II which is a critical regulator of blood pressure and electrolyte balance, is constitutively produced by the liver and secreted into the circulation. The 5'-flanking region of the human angiotensinogen gene has been found to regulate its own expression in the liver of transgenic mice, but the transcriptional regulation of the human angiotensinogen gene has not been reported. We analyzed its promoter region by utilizing the reporter plasmids that contain the 5'-flanking region with various lengths fused to the bacterial chloramphenicol acetyltransferase (CAT) gene. In transiently transfected human hepatoma HepG2 cells, the only 32-bp DNA region located at nucleotide positions between -344 and -313 bp was shown to mediate half as much as the full extent of parental promoter activity. An electrophoretic mobility shift assay (EMSA) using this segment identified the formation of DNA/HepG2 nuclear factor complexes. These complexes could be dissociated by competing recognition sequences that contain those of either Sp1,a widely expressed GC box-binding factor, or RBF-1,a recently characterized GC box-binding factor regulating the human retinoblastoma (Rb) gene. An in vivo competition experiment also suggested that the CAT activity derived from the cotransfected angiotensinogen promoter is reduced by a competitor containing RBF-1 binding sites or containing Sp1 binding sites. These findings suggest that Sp1 and RBF-1 play an important role in maintaining the constitutive promoter activity of the human angiotensinogen gene and that the angiotensinogen gene is characterized as the second target for RBF-1 beside the Rb gene.

• 近畿大学の論文

著者

• Shimada Seijiro

  First Department of Internal Medicine, Kinki University School of Medicine

• Shimada Seijiro

  Department Of Cardiology Sakai Hospital Kinki University School Of Medicine

• Shimada Seijiro

  First Department Of Internal Medicine Kinki University School Of Medicine

関連論文

• EFFECT OF AN ANGIOTENSIN II RECEPTOR ANTAGONIST, TCV-116, ON RAT CAROTID ARTERY NEOINTIMA FORMATION AFTER BALLOON INJURY
• ANTISENSE ODNS FOR C-MYC MRNA PRODUCED SIGNIFICANT INHIBITION IN THE HYPERTROPHIC EFFECT OF ANGIOTENSIN II ON VASCULAR SMOOTH MUSCLE CELLS
• ATRIAL NATRIURETIC PEPTIDE AND ANTIHYPERTENSIVE ACTION DUE TO β-BLOCKADE IN ESSENTIAL HYPERSIVE PATIENTS
• HYPERTROPHIC EFFECT OF ANGIOTENSIN II ON VACULAR SMOOTH MUSCLE CELLS IS INDUCED BY C-MYC PRODUCTION
• Monocyte Count is a Risk Factor for Subclinical Atherosclerosis in Hypertension(Atherosclerosis, Clinical 2 (IHD), The 69th Annual Scientific Meeting of the Japanese Circulation Society)
• Marked hypercalcemia and weight loss in a patient with myocardial infarction complicated by psoriasis vulgaris
• Infectious Endocarditis without Marked Signs of Inflammation where High Sensitivity CRP Assay was Effective
• -P521- EFFECTS OF AN ANGIOTENSIN II RECEPTOR ANTAGONIST, TCV-116, ON NEOINTIMAL FORMATION FOLLOWING BALLOON INJURY IN SHR CAROTID ARTERY(PROCEEDINGS OF THE 59th ANNUAL SCIENTIFIC MEETING OF THE JAPANESE CIRCULATION SOCIETY)
• Fever Associated With Acute Aortic Dissection
• PJ-879 Carotid Intima-Media Thickness Reflects the Synergistic Progression of Arteriosclerosis in Whole Generations of Patients with both Hypertension and Diabetes(Hypertension, clinical(10)(H),Poster Session(Japanese),The 72nd Annual Scientific Meeting o
• P671 EFFECTS OF THE ANGIOTENSIN-CONVERTING ENZYME INHIBITOR IN CARDIAC HYPERTROPHY IN TSUKUBA
• PJ-647 Carotid Intima-Media Thickness but not Pulse Wave Velocity Reflects the Synergistic Progression of Arteriosclerosis in Diabetic Patients with Hypertension(Hypertension, clinical-09, The 71st Annual Scientific Meeting of the Japanese Circulation Soc
• Inhibitory Effects of a Subdepressor Dose of L-158, 809, an Angiotensin II Type 1 Receptor Antagonist, on Cardiac Hypertrophy and Nephropathy Via the Activated Human Renin-Angiotensin System in Double Transgenic Mice With Hypertension
• Two distinct nuclear factors, Sp1 and RBF-1,maintaining constitutive promoter activity of human angiotensinogen gene

もっと見る 閉じる

スポンサーリンク