<Originals>Genetic alterations in sporadic colorectal carcinomas at all clinical stages of progression
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概要
- 論文の詳細を見る
The accumulation of genetic alterations during the adenoma-carcinoma sequence is associated with the development and/or progression of colorectal carcinoma. To examine whether alterations in APC, K-ras, and p53 genes, and allelic loss of 18q play a role in the late stage of progression of colorectal carcinoma, DNA samples from 40 patients with colorectal carcinoma at all clinical stages (7 Dukes A, 14 Dukes B, 19 Dukes C) were studied for loss of heterozygosity (LOH) at the loci on chromosomes 5q21,17p13 and 18q21,using restriction fragment length polymorphism analysis. In addition, mutations in the APC, K-ras and p53 genes were examined by RNase protection analysis and subsequent sequencing analysis. LOH at 5q21,17p13 and 18q21 was identified in 31%, 74%, and 59% of the informative tumor samples, respectively. Somatic mutations in APC, K-ras, and p53 gene were detected in 38%, 38%, and 53% of the 40 carcinomas, respectively. There was a significant correlation between genetic alterations in the APC and K-ras genes (p<0.01). With progressive Dukes'stages, the frequency of genetic alterations increased. Five of 19 Dukes C tumors showed more than three genetic alterations. However, only one Dukes A tumor had three alterations. These findings suggest that tumor cells with an increased number of genetic alterations may contribute to malignant progression in colorectal carcinoma.
- 近畿大学の論文
著者
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WATATANI Masahiro
First Department of Surgery, Kinki University School of Medicine
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INUI HIROKI
First Department of Surgery, Kinki University School of Medicine
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Inui H
Department Of Surgery Kinki University School Of Medicine
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Inui Hiroki
First Department Of Surgery Kinki University School Of Medicine
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Watatani M
Department Of Surgery Kinki University School Of Medicine
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Watatani Masahiro
First Department Of Surgery Kinki University School Of Medicine
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