<Originals>An evaluation of myocardial protection in non hypertensive rats (WKY) and spontaneously hypertensive rats (SHR) by 31-phosphorus nuclear magnetic resonance
スポンサーリンク
概要
- 論文の詳細を見る
The effect of myocardial protection on energy metabolism was studied in the hearts of Wistar Kyoto rats (WKY) and spontaneously hypertensive rats (SHR). Isolated rat heart was perfused via the aorta by the Langendorff method and cardiac arrest was induced by modified Young's solution. High energy phosphate compounds and intracellular pH were nondestructively measured by 31-phosphorus nuclear magnetic resonance spectroscopy. In the first series of experiments, hypothermic ischemia was induced at 25℃, 15℃ and 4℃ (control 37℃) for 60 minutes in the hearts of WKY. After 30 minutes of ischemia at 37℃ and 60 minutes of ischemia at 25℃, the adenosine triphosphate (ATP) and the intracellular pH fell to approximately 20% of the initial one (absolute value 1.2 mM) and 6.3,respectively. These values for time and temperature were considered the critical level. The ATP and intracellular pH in 4℃ group were significantly higher than the other three groups. In the second series of experiments, the hearts of WKY and SHR were treated by three different cardioplegic methods and subjected 90 minutes of ischemia at 15℃ and reperfusion at 37℃ : topical hypothermia alone (group I, control group) and the following cardioplegic solutions were intermittently infused every 15 minutes ; glucose insulin potassium cardioplegia (group II), cold blood cardioplegia (group III), and same as group III with administration of coenzyme Q_<10> prior to the isolation of heart (group IV). In WKY heart, after 90 minutes of ischemia, the ATP in group IV was significantly higher than the other groups except group III. After 30 minutes of reperfusion, the creatine phosphate (CrP) and the intracellular pH returned to the initial level in all the groups, but the ATP returned only to 75±6% of the initial level even in group IV in which the value was significantly higher than that in the other three groups. In the groups of SHR, the initial value of ATP was similar to and that of CrP was approximately 50% of the level in WKY. The control value of intracellular pH in SHR groups was 7.0±0.15,which was significantly lower than that in WKY (7.2±0.08) (p<0.01). After 90 minutes of ischemia, even in group IV, the ATP, CrP and intracellular pH were 41%, 0% and 6.7,and after reperfusion 27%, 41% and 6.8,respectively. In conclusion, the use of coenzyme Q_<10> provided the best myocardial protection in the hearts of WKY and SHR, however, the effect was not recognized in SHR during reperfusion, compared with that in WKY. In SHR hearts, though the value of ATP was equal to that in WKY, the values of CrP and intracellular pH were significantly lower. The values of ATP and intracellular pH were over the critical level, however, the ATP showed a tendency to decrease rather than to increase and the recovery of the CrP and intracellular pH was inadequate during reperfusion. These suggest that the heart of SHR being ischemic state and of great importance is considered reperfusion injury rather than ischemic one.
- 近畿大学の論文
著者
関連論文
- The effect of neuronal perturbation on the uptake of [^F]2-fluoro-2-deoxy-D-glucose in brain slices of the rat
- Renal Preservation in Low Ectopic Right Renal Artery Reconstruction During Abdominal Aortic Aneurysm Repair : Report of a Case
- Intravascular Extraction of Permanent Pacemaker Leads
- 当院における10年間の上部消化管出血治療の臨床的検討
- RECONSTRUCTION OF PULMONARY ARTERY WITH SUBSTITUTE VALVE : With Special Reference to Size of Conduit and Valve
- SURGICAL CORRECTION OF VALVE DISEASE IN THE CHILDREN : Congential Heart Disease : 46th Annual Scientific Meeting, Japanese Circulation Society
- SURGICAL RESULTS AND FACTORS AFFECTING OPERATIVE MORTALITY IN TOTAL ANOMALOUS PULMONARY VENOUS DRAINAGE
- ANATOMICALLY CORRECTED MALPOSITION OF THE GREAT ARTERIES : Case Reports and a Review
- Successful Surgical Repair of Corrected Transposition of the Great Arteries (S, L, L, ) with Ventricular Septal Defect and Regurgitation of the Left Atrioventricular Valve. A Case Report
- Two Cases of a Free-Floating Ball Thrombus in the Left Atrium
- Three-dimensional Recovery Time Dispersion Map of Maximum dV/dt in The ST Segment by Magnetocardiography May Demonstrate a Injured Myocardium(ECG/Body Surface Potential Mapping/Holter 3 (A), The 69th Annual Scientific Meeting of the Japanese Circulation S
- Panic disorder with and without agoraphobia : comorbidity within a half-year of the onset of panic disorder
- Coronary Artery Bypass Grafting in Octogenarians
- Obesity as a risk factor for GERD in Japan
- Developmental changes in the electrophysiological properties of neonatal rat oculomotor neurons studied in vitro
- Topographical projections from the medial septum-diagonal band complex to the hippocampus: a retrograde tracing study with multiple fluorescent dyes in rats
- An evaluation of myocardial protection in non hypertensive rats (WKY) and spontaneously hypertensive rats (SHR) by 31-phosphorus nuclear magnetic resonance
- Irradiation Effect on Tensile Property of F82H IEA and Its Joint in TITAN Project