A Novel Reductase from Candida albicans for the Production of Ethyl (S)-4-Chloro-3-hydroxybutanoate
スポンサーリンク
概要
- 論文の詳細を見る
A novel NADPH-dependent reductase (CaCR) from Candida albicans was cloned for the first time. It catalyzed asymmetric reduction to produce ethyl (S)-4-chloro-3-hydroxybutanoate ((S)-CHBE). It contained an open reading frame of 843 bp encoding 281 amino acids. When co-expressed with a glucose dehydrogenase in Escherichia coli, recombinant CaCR exhibited an activity of 5.7 U/mg with ethyl 4-chloro-3-oxobutanoate (COBE) as substrate. In the biocatalysis of COBE to (S)-CHBE, 1320 mM (S)-CHBE was obtained without extra NADP+/NADPH in a water/butyl acetate system, and the optical purity of the (S)-isomer was higher than 99% enantiomeric excess.
- Japan Society for Bioscience, Biotechnology, and Agrochemistryの論文
- 2012-06-23
著者
-
Cai Ping
College Of Biotechnology And Pharmaceutical Engineering Nanjing University Of Technology
-
Hao Ning
College Of Biotechnology And Pharmaceutical Engineering Nanjing University Of Technology
-
Liu Huan
College Of Biotechnology And Pharmaceutical Engineering Nanjing University Of Technology
-
Li Yan
College Of Biotechnology And Pharmaceutical Engineering Nanjing University Of Technology
-
Yan Ming
College Of Biotechnology And Pharmaceutical Engineering Nanjing University Of Technology
-
AN Mingdong
College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology
-
WANG Shanshan
College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology
-
XU Lin
College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology
-
An Mingdong
College Of Biotechnology And Pharmaceutical Engineering Nanjing University Of Technology
-
Xu Lin
College Of Biotechnology And Pharmaceutical Engineering Nanjing University Of Technology
-
Wang Shanshan
College Of Biotechnology And Pharmaceutical Engineering Nanjing University Of Technology
-
LI Yan
College of Asia Pacific Study Ritsumeikan Asia Pacific University
関連論文
- Effects of Fermentation Temperature on the Content and Composition of Isoflavones and β-Glucosidase Activity in Sufu
- A Simple Iodine-promoted Synthesis of 2-Substituted Benzothiazoles by Condensation of Aldehydes with 2-Aminothiophenol
- A Novel Reductase from Candida albicans for the Production of Ethyl (S)-4-Chloro-3-hydroxybutanoate
- Techniques of Electromagnetic Compatibility Model Synthesis Based on On-Site Measurement Data
- Bioconversion of Phosphatidylserine by Phospholipase D from Streptomyces racemochromogenes in a Microaqueous Water-Immiscible Organic Solvent
- RELATIONSHIPS BETWEEN NETWORK TOPOLOGY AND PEDESTRIAN ROUTE CHOICE BEHAVIOR