Rapid and Economic DNA Extraction from a Single Salmon Egg for Real-Time PCR Amplification

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概要

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• Salmon eggs are common in Japanese sushi and other seafood products; however, certain fish eggs are used as counterfeit salmon eggs which are found in foods and processed products. This study develops a simple, rapid, and cost-effective method for DNA extraction, filtration (FT) and dilution (DL) protocols from a single salmon egg with good DNA quality for real-time PCR amplification. The DNA amount, DNA quality, and real-time PCR performance for different dilutions and different lengths of PCR amplicons were evaluated and compared with the common Qiagen tissue kit (QTK) and Chelex-100-based (CX) protocols. The extracted DNA from a single salmon egg using the FT or DL protocol can be applied in phylogenic research, food authentication and post-marketing monitoring of genetically modified (GM) food products.

• 2011-10-23

著者

• Yang Jing-iong

  Department Of Seafood Science National Kaohsiung Marine University

• Chou Yii-cheng

  Department Of Life Sciences National Tsing Hua University

• HUANG Hsiao-Yun

  Department of Seafood Science, National Kaohsiung Marine University

• CHEN Chien-Cheng

  Department of Biotechnology, National Kaohsiung Normal University

• LEE Guo-Chi

  Department of Science and Technology, Council of Agriculture

• Chen Chien-cheng

  Department Of Biotechnology National Kaohsiung Normal University

• Chang Hsueh-wei

  Department Of Biomedical Science And Environmental Biology Graduate Institute Of Natural Products Co

• Huang Hsiao-yun

  Department Of Seafood Science National Kaohsiung Marine University

• Lee Guo-chi

  Department Of Science And Technology Council Of Agriculture

• Chou Yii-cheng

  Department Of Medical Laboratory Science And Biotechnology Chung Hwa University Of Medical Technolog

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