Immunohistochemical Localization of Endogenous D-Aspartate in the Marine Brown Alga Sargassum fusiforme
スポンサーリンク
概要
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Immunohistochemical localization (cellular localization) of endogenous D-aspartate in the marine brown alga Sargassum fusiforme was investigated by the use of a specific polyclonal antibody raised against D-aspartate. D-Aspartate immunoreactivity was evident in the medullary layer in the blade of the alga, and weak staining was found in the cortical layer, whereas epidermal cells were found to lack D-aspartate. Within the cells of the layers, immunoreactivity was confirmed only in the cytosol and not in the cell wall, chloroplast, or vacuole. These results suggest that D-aspartate is present in S. fusiforme cells, and excludes the possibility that it is derived from attached or symbiotic organisms such as marine bacteria. This is the first report describing the localization of free D-aspartate in plant cells.
- 2011-08-23
著者
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Amano Masafumi
School Of Fisheries Sciences Kitasato University Sanriku
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YOKOYAMA Takehiko
School of Fisheries Science, Kitasato University
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Amano Masafumi
School Of Marine Biosciences Kitasato University
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Sato Minoru
Graduate School Of Agricultural Science Tohoku University
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Homma Hiroshi
Department Of Architecture And Civil Engineering Toyohashi University Of Technology
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SEKINE Masae
Department of Pharmaceutical Life Sciences, Kitasato University
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TOKUDA Masaharu
National Research Institute of Aquaculture, Fisheries Research Agency
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Sato Minoru
Graduate School Of Agriculture Tohoku University
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Homma Hiroshi
Department Of Pharmaceutical Life Sciences Kitasato University
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Sekine Masae
Department Of Pharmaceutical Life Sciences Kitasato University
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Tokuda Masaharu
National Research Institute Of Aquaculture Fisheries Research Agency
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