An Updated Loop-Mediated Isothermal Amplification Method for Rapid Diagnosis of H5N1 Avian Influenza Viruses
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概要
- 論文の詳細を見る
We designed a new set of primers for reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) to specifically amplify the HA gene of avian influenza viruses subtype H5N1. By testing nine H5N1 virus strains and 41 clinical samples collected in Northern Vietnam, we found that the new primers showed higher sensitivity and specificity than the previously published RT-LAMP primers and were comparable to the RT-PCR method currently recommended by WHO. These results suggest that our RT-LAMP assay may be a better choice as a diagnostic tool for current H5N1 influenza virus infection.
- Japanese Society of Tropical Medicine,日本熱帯医学会の論文
- 2011-03-01
著者
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Hasebe Futoshi
Department Of Virology Institute Of Tropical Medicine
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Morita Kouichi
Department Of Virology Institute Of Tropical Medicine Nagasaki University
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Le Mai
Department Of Virology National Institute Of Hygiene And Epidemiology
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Quynh Le
Department Of Virology National Institute Of Hygiene And Epidemiology
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DINH Duc
Department of Virology, Institute of Tropical Medicine, GCOE Program, Nagasaki University
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VUONG Cuong
Department of Virology, National Institute of Hygiene and Epidemiology
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Vuong Cuong
Department Of Virology National Institute Of Hygiene And Epidemiology
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Hasebe Futoshi
Department Of Virology Institute Of Tropical Medicine Gcoe Program Nagasaki University
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Morita Kouichi
Department Of Virology Institute Of Tropical Medicine Gcoe Program Nagasaki University
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Dinh Duc
Department Of Virology Institute Of Tropical Medicine Gcoe Program Nagasaki University
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