Plantlet regeneration from protoplasts of Muscari armeniacum Leichtl. ex Bak

スポンサーリンク

概要

• 論文の詳細を見る

• Protoplasts were isolated from embryogenic calluses of Muscari armeniacum ‘Blue Pearl’, which had been subcultured for 3 years. Protoplasts started to divide after 5–7 days of culture, and colonies consisting of 50–100 cells were produced after one month. The highest plating efficiency (10.9%) was obtained by using a medium containing 5.4 µM NAA and 4.4 µM BA, 0.5 M glucose and 2 g l−1 gellan gum. Protoplast-derived calluses produced somatic embryos at frequencies of 4.3–89.6% on media containing 0 or 0.54 µM NAA in combination with 0, 4.4, 22 or 44 µM BA, but few embryos converted into plantlets. On the other hand, over 35% of the calluses produced adventitious shoots on media containing 4.4 µM BA or 0.54 µM NAA in combination with 44 µM BA, and some of these shoots developed into plantlets following transfer to a medium without PGRs.

• 日本植物細胞分子生物学会の論文
• 2005-09-01

著者

• NAKANO Masaru

  Faculty of Agriculture, Niigata University

• Nakano Masaru

  Faculty Of Agriculture Niigata University

• Tanaka Shigefumi

  Faculty of Agriculture, Niigata University

• KAGAMI Shiho

  Faculty of Agriculture, Niigata University

• SAITO Hiroyuki

  Plant Functions Laboratory, RIKEN (The Institute of Physical and Chemical Research)

• Tanaka Shigefumi

  Faculty Of Agriculture Niigata University

• Saito Hiroyuki

  Plant Functions Laboratory Riken (the Institute Of Physical And Chemical Research)

• Kagami Shiho

  Faculty Of Agriculture Niigata University

関連論文

• Morphological variation in Tricyrtis hirta plants regenerated from heavy ion beam-irradiated embryogenic calluses
• Interspecific hybridization between triploid Senno (Lychnis senno Siebold et Zucc., Caryophyllaceae) and allied taxa of the genus Lychnis
• Adventitious shoot regeneration and micropropagation of the Japanese endangered Hylotelephium sieboldii (Sweet ex Hook.) H. Ohba and H. sieboldii var. ettyuense (Tomida) H. Ohba
• Intergeneric hybridization among colchicaceous ornamentals, Gloriosa spp., Littonia modesta and Sandersonia aurantiaca via ovule culture
• Early Identification of Intra- and Intergeneric Hybrids among Colchicaceous Ornamentals, Gloriosa spp., Littonia modesta Hook. and Sandersonia aurantiaca Hook., by Flow Cytometry and Random Amplified Polymorphic DNA Analyses
• PRODUCTION OF ALTERNARIA BLOTCH DISEASE-RESISTANT APPLE BY TRANSFORMATION OF A FUNGAL CHITINASE
• Partial Synchronization of Cell Division and Micronucleation in Suspension-cultured Cells of Hemerocallis hybrida : The effects of Hydroxyurea and Various Spindle Toxins
• Flow Cytometric Analysis of Partially Synchronized Suspension Cultures of Hemerocallis hybrida
• Recent advances in wide hybridization and embryo rescue of floricultural plants in Japan
• AN INTEGRATED ANALYTICAL/SIMULATION APPROACH FOR ECONOMIC DESIGN OF AN AGV SYSTEM
• Effects of Several Antibiotics and Bialaphos on the Growth and Organ Formation of Lilium formosanum Calli and Transient Expression of the gusA Gene after Co-cultivation with Agrobacterium tumefaciens
• Adventitious shoot regeneration and micropropagation of the Japanese endangered Hylotelephium sieboldii (Sweet ex Hook.) H. Ohba and H. sieboldii var. ettyuense (Tomida) H. Ohba
• Plantlet regeneration from protoplasts of Muscari armeniacum Leichtl. ex Bak
• Isolation and Characterization of Microprotoplasts from Propyzamide-treated Cell Suspension Cultures of Hemerocallis hybrida

もっと見る 閉じる

スポンサーリンク