New Frontiers in Gut Nutrient Sensor Research : Prophylactic Effect of Glutamine Against Helicobacter pylori-Induced Gastric Diseases in Mongolian Gerbils
スポンサーリンク
概要
- 論文の詳細を見る
Ammonia is one of the important toxins produced by Helicobacter pylori (H. pylori), the major cause of peptic ulcer diseases. We examined whether glutamine or marzulene (a gastroprotective drug containing 1% sodium azulene and 99% glutamine) protects the gastric mucosa against H. pylori in vivo and investigated the mechanism underlying glutamine-induced mucosal protection against ammonia in gastric epithelial cells in vitro. Mongolian gerbils were fed for 3 months with a diet containing glutamine (2% – 20%) or marzulene (20%) starting from 2 weeks or 2 years after H. pylori infection. Then, gastric mucosal changes were evaluated both macro- and microscopically. Cultured gastric epithelial cells were incubated in the presence of ammonia, with or without glutamine; and cell viability, ammonia accumulation, and chemokine production were determined. Gerbils exhibited edema, congestion, and erosion after 3-month infection; and after 2-year infection, they showed cancer-like changes in the gastric mucosa. Glutamine and marzulene significantly suppressed these pathological changes caused in the gastric mucosa by H. pylori infection. Ammonia was accumulated in the cells, resulting in an increase in chemokine production and a decrease in cell viability. These pathological responses were prevented by glutamine. In addition, glutamine decreased chemokine production and cell death through inhibition of cellular accumulation of ammonia, resulting in the prevention of H. pylori–induced gastric diseases in vivo. These results suggest that glutamine/marzulene would be useful for prophylactic treatment of H. pylori–induced gastric diseases in patients.
- 社団法人 日本薬理学会の論文
- 2010-01-20
著者
-
Hasumura Mai
Physiology & Nutrition Group, Institute of Life Sciences, Ajinomoto Co., Inc., Japan
-
Uneyama Hisayuki
Physiology & Nutrition Group, Institute of Life Sciences, Ajinomoto Co., Inc., Japan
-
Torii Kunio
Physiology & Nutrition Group, Institute of Life Sciences, Ajinomoto Co., Inc., Japan
-
Takeuchi Koji
Division of Pathological Sciences, Department of Pharmacology and Experimental Therapeutics, Kyoto P
-
Uneyama Hisayuki
Physiology And Nutrition Group Institute Of Life Sciences Ajinomoto Co. Inc.
-
Torii Kunio
Ajinomoto Co. Inc. Kawasaki Jpn
-
Torii Kunio
Inst. Of Life Sciences Ajinomoto Co. Inc.
-
Nakamura Eiji
Division of Pathological Sciences, Department of Pharmacology and Experimental Therapeutics, Kyoto P
-
Endo Takuya
Division of Pathological Sciences, Department of Pharmacology and Experimental Therapeutics, Kyoto P
-
Hayashi Shusaku
Division of Pathological Sciences, Department of Pharmacology and Experimental Therapeutics, Kyoto P
-
Nakamura Eiji
Physiology & Nutrition Group Institute Of Life Sciences Ajinomoto Co. Inc.
-
Amagase Kikuko
Div. Of Pathological Sciences Dep. Of Pharmacology And Experimental Therapeutics Kyoto Pharmaceutica
-
Amagase Kikuko
Division Of Pathological Sciences Department Of Pharmacology And Experimental Therapeutics Kyoto Pha
-
Hasumura Mai
Physiology & Nutrition Group Institute Of Life Sciences Ajinomoto Co. Inc.
-
Takeuchi Koji
Division Of Pathological Sciences Department Of Pharmacology And Experimental Therapeutics Kyoto Pha
-
Endo Takuya
Division Of Pathological Sciences Department Of Pharmacology And Experimental Therapeutics Kyoto Pha
-
Hayashi Shusaku
Division Of Pathological Sciences Department Of Pharmacology And Experimental Therapeutics Kyoto Pha
-
Takeuchi Koji
Department Of Pharmacology And Experimental Therapeutics Kyoto Pharmaceutical University.
-
Uneyama Hisayuki
Inst. For Innovation Ajinomoto Co. Inc. Jpn
関連論文
- New Frontiers in Gut Nutrient Sensor Research: Luminal Glutamate–Sensing Cells in Rat Gastric Mucosa
- Prostaglandin EP receptors involved in modulating gastrointestinal mucosal integrity
- Prostaglandin EP Receptors Involved in Modulating Gastrointestinal Mucosal Integrity
- 1999年度日本味と匂学会第33回大会
- 1999年度日本味と匂学会第33回大会
- Aggravation of Cold-Restraint Stress-Induced Gastric Lesions in Adjuvant Arthritic Rats : Pathogenic Role of Inducible and Endothelial Nitric Oxide
- EFFECT OF GLUCOCORTICOID DEFICIENCY ON INDOMETHACIN-INDUCED GASTRIC MICROVASCULAR PERMEABILITY, HYPERMOTILITY AND GASTRIC MUCOSAL INJURY IN RATS
- Alteration of Gastric Ulcerogenic and Healing Responses in Rats With Adjuvant-Induced Arthritis
- Protective Effect of Thiaton an Antispasmodic Drug, Against Indomethacin-Induced Intestinal Damage in Rats
- Compensatory gastroprotective role of glucocorticoid hormones during inhibition of prostaglandins, nitric oxide and desensitization of capsaicin-sensitive neurons
- On theMechanisms Underlying Histamine Induction of Gastric Mucosal Lesions in Rats With Partial Gastric Vascular Occlusion
- New Frontiers in Gut Nutrient Sensor Research: Prophylactic Effect of Glutamine Against Helicobacter pylori–Induced Gastric Diseases in Mongolian Gerbils
- Role played by afferent signals from olfactory, gustatory and gastrointestinal sensors in regulation of autonomic nerve activity (Taste and health: nutritional and physiological significance of taste substances in daily foods)
- Aggravation of Cold-Restraint Stress–Induced Gastric Lesions in Adjuvant Arthritic Rats: Pathogenic Role of Inducible and Endothelial Nitric Oxide
- Physiological Roles of Dietary Free Glutamate in Gastrointestinal Functions(Physiology and Pharmacology of the Gut Nutrient Perception)
- Dopamine D2–Receptor Antagonists Ameliorate Indomethacin-Induced Small Intestinal Ulceration in Mice by Activating α7 Nicotinic Acetylcholine Receptors
- New Frontiers in Gut Nutrient Sensor Research : Prophylactic Effect of Glutamine Against Helicobacter pylori-Induced Gastric Diseases in Mongolian Gerbils
- New Frontiers in Gut Nutrient Sensor Research : Luminal Glutamate-Sensing Cells in Rat Gastric Mucosa
- Brain Activation by Umami Substances via Gustatory and Visceral Signaling Pathways, and Physiological Significance(Physiology and Pharmacology of the Gut Nutrient Perception)
- Effects of intragastric infusion of inosine monophosphate and L-glutamate on vagal gastric afferent activity and subsequent autonomic reflexes
- New Frontiers in Gut Nutrient Sensor Research-From Taste Physiology to Gastroenterology : Preface
- Dopamine D_2-Receptor Antagonists Ameliorate Indomethacin-Induced Small Intestinal Ulceration in Mice by Activating α7 Nicotinic Acetylcholine Receptors
- Prostaglandin EP Receptors Involved in Modulating Gastrointestinal Mucosal Integrity
- New Therapeutic Strategy for Amino Acid Medicine : Effects of Dietary Glutamate on Gut and Brain Function
- New Therapeutic Strategy for Amino Acid Medicine : Prophylactic and Healing Promoting Effect of Monosodium Glutamate Against NSAID-Induced Enteropathy
- Activation of Muscarinic Acetylcholine Receptor Subtype 4 is Essential for Carbachol-Induced Acid Secretion in Mice : Relation to D Cells/Somatostatin
- New Therapeutic Strategy for Amino Acid Medicine : Preface
- Foreword