Comparison of Commercial ELISA Blood Tests for Early Pregnancy Detection in Dairy Cows

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概要

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• The objective of the present study was to compare two commercially available blood-based pregnancy tests, namely BioPRYN, an ELISA for pregnancy-specific protein B (PSPB), and an ELISA for pregnancy-associated glycoprotein (PAG), for early pregnancy diagnosis in dairy cattle using transrectal ultrasonography as a gold standard. Transrectal ultrasonography was conducted 26-58 days after artificial insemination (AI) in 197 cattle from 19 farms. Concurrently, a blood sample was collected for determination of serum PSPB and PAG. Transrectal palpation was performed approximately 120 days after AI to verify that pregnancy was maintained. For PSPB and PAG, there were no significant differences (P>0.05) in sensitivity (98.0 and 97.8%), specificity (97.1 and 91.2%), positive predictive values (99.3 and 97.8%), negative predictive values (91.9 and 91.2%) and accuracy (97.8 and 96.4%). In conclusion, the two blood pregnancy assays were equally efficacious and were highly accurate (based on transrectal ultrasonography as the gold standard).

• 2011-02-01

著者

• Bollwein Heinrich

  Clinic For Cattle University Of Veterinary Medicine Hannover

• Sasser Garth

  Biotracking Llc

• Bollwein Heinrich

  Clinic For Cattle School Of Veterinary Medicine Hannover

• Bollwein Julia

  Clinic For Cattle University Of Veterinary Medicine Hannover

• Friedrich Morten

  Department Of Animal Science University Of Gottingen

• Piechotta Marion

  Clinic For Cattle University Of Veterinary Medicine Hannover

• HEILKENBRINKER Thomas

  Animal Health Department, Chamber of Agriculture Lower Saxony

• PASSAVANT Chuck

  BioTracking, LLC

• BRANEN Josh

  BioTracking, LLC

• HOEDEMAKER Martina

  Clinic for Cattle, University of Veterinary Medicine Hannover

• Branen Josh

  Biotracking Llc

• Hoedemaker Martina

  Clinic For Cattle University Of Veterinary Medicine Hannover

• Heilkenbrinker Thomas

  Animal Health Department Chamber Of Agriculture Lower Saxony

• Passavant Chuck

  Biotracking Llc

関連論文

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• Genital Blood Flow and Endometrial Gene Expression During the Preovulatory Period after Prostaglandin F_-Induced Luteolysis in Different Luteal Phases in Cows
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• Testicular Blood Flow and Plasma Concentrations of Testosterone and Total Estrogen in the Stallion after the Administration of Human Chorionic Gonadotropin
• Comparison of Commercial ELISA Blood Tests for Early Pregnancy Detection in Dairy Cows
• Relationships Between Uterine Blood Flow, Peripheral Sex Steroids, Expression of Endometrial Estrogen Receptors and Nitric Oxide Synthases During the Estrous Cycle in Mares
• Gene Expressions in the Persistent Corpus Luteum of Postpartum Dairy Cows : Distinct Profiles from the Corpora Lutea of the Estrous Cycle and Pregnancy
• Downregulation of Lymphatic Vessel Formation Factors in PGF2α-induced Luteolysis in the Cow

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