Detection and identification of rumen bacteria constituting a fibrolytic consortium dominated by Fibrobacter succinogenes
スポンサーリンク
概要
- 論文の詳細を見る
A fibrolytic consortium, dominated by the rumen cellulolytic bacterium Fibrobacter succinogenes, was artificially constructed on hay stems to detect and identify rumen bacteria that can potentially interact with F. succinogenes. Consortium-bacterial members were determined by DGGE and sequencing analysis targeted bacterial 16S rDNA. An artificial consortium was formed in a 2-step incubation of hay stems; the first step with group 1, 2 or 3 F. succinogenes strains, the second step with rumen fluid. After consortium formation, morphologically different bacteria were observed in association with F. succinogenes. DGGE exhibited more than 30 bands, the pattern of which depended on the F. succinogenes group. Sequencing suggested that Butyrivibrio fibrisolvens, Pseudobutyrivibrio ruminis, Clostridium sp., F. succinogenes group 2, Prevotella ruminicola and unclassified Bacteroides were prominent in the group 1 consortium and that Treponema bryantii, B. fibrisolvens, Acinetobacter sp, and Wolinella succinogenes were prominent in the group 2 consortium. However, in the group 3 consortium, F. succinogenes-like bacteria were microscopically undetectable, whereas cellulolytic Ruminococcus albus and F. succinogenes group 1 were prominent, suggesting that the group 3 cannot be a core member of this consortium. This study is the first attempt to identify bacterial members of a fibrolytic consortium dominated by a specific bacterium.ルーメン内繊維分解性細菌Fibrobacter succinogenesと相互作用をもつ細菌種を検出・同定するため、本菌系統グループ1から3の菌株を中心とする繊維分解コンソーシャムをオーチャードグラス乾草上に人為的に作成した（作成コンソーシャム）。作成コンソーシャムにおいてはF. succinogenes様細胞に異なる形態の細菌が付着する様子が電顕観察された。作成コンソーシャムのDGGE解析では30以上のバンドが検出され、そのバンドパターンは主構成員である系統グループの違いを反映していた。グループ1の作成コンソーシャムからはButyrivibrio fibrisolvens, Pseudobutyrivibrio ruminis, Clostridium属細菌, F. succinogenesグループ2, Prevotella ruminicolaおよび未培養のBacteroides属細菌が、グループ2のそれからはTreponema bryantii, B. fibrisolvens, Acinetobacter属細菌およびWolinella succinogenesが構成細菌として同定された。一方、グループ3のコンソーシャムでは主構成員であるはずのF. succinogenesの観察頻度は下がり、他の繊維分解菌に置き換わっていたため、グループ3のF. succinogenesはコンソーシャムで主導的な役割を担い得ないと考えられた。本研究は特定の細菌を中心として構成される繊維分解コンソーシャムに対し、その構成細菌を明らかにしようとした初めての報告である。
- 2010-02-01
著者
-
Kobayashi Yasuo
Graduate School Of Agriculture Hokkaido University
-
Ueki Takaaki
Graduate School Of Agriculture Hokkaido University
-
Shinkai Takumi
Graduate School Of Agriculture Hokkaido University
関連論文
- Inclusion of novel bacteria in rumen microbiology : Need for basic and applied science
- Rumen microbes and fermentation of wild sika deer on the Shiretoko peninsula of Hokkaido Island, Japan
- Fiber digestion in various segments of the hindgut of horses fed grass hay or silage
- Mean retention time of digesta in the different segments of the equine hindgut
- Hindgut microbes, fermentation and their seasonal variations in Hokkaido native horses compared to light horses
- Ruminal distribution of the cellulolytic bacterium Fibrobacter succinogenes in relation to its phylogenetic grouping
- Phylogenetic analysis of hindgut microbiota in Hokkaido native horses compared to light horses
- Effects of lauric acid on physical, chemical and microbial characteristics in the rumen of steers on a high grain diet
- Effects of supplemental lauric acid-rich oils in high-grain diet on in vitro rumen fermentation
- Stable Production of Thermotolerant Xylanase B of Clostridium stercorarium in Transgenic Tobacco and Rice
- Detection and identification of rumen bacteria constituting a fibrolytic consortium dominated by Fibrobacter succinogenes
- Ecological characterization of three different phylogenetic groups belonging to the cellulolytic bacterial species Fibrobacter succinogenes in the rumen
- Partial characterization of structure and function of a xylanase gene from the rumen hemicellulolytic bacterium Eubacterium ruminantium
- Validation and application of real-time polymerase chain reaction assays for representative rumen bacteria
- CONVERSION OF UNUTIRIZED BIOMASS TO FEED AND FUEL BY ANAEROBIC BACTERIA
- Synergistic fibrolysis in the rumen by cellulolytic Ruminococcus flavefaciens and non-cellulolytic Selenomonas ruminantium : Evidence in defined cultures
- Imaging of Time and Space Variation of Vortex Wind Velocity Fields Using Acoustic Tomography