Vacuum-Ultraviolet Circular Dichroism Analysis of Glycosaminoglycans by Synchrotron-Radiation Spectroscopy
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概要
- 論文の詳細を見る
Glycosaminoglycans (GAGs) are important polysaccharides with various biological functions, but their structures in solution are affected in a complex manner by their component residues. We successfully measured the vacuum-ultraviolet circular dichroism (VUVCD) spectra of six GAGs (chondroitin, chondroitin sulfates, hyaluronic acid, and heparin) and their component sugars (N-acetylaminosugars and uronic acid) from 240 to 160 nm by a synchrotron-radiation VUVCD spectrophotometer in aqueous solutions at 25 °C. These comprehensive VUVCD spectra revealed the characteristic contribution of the constituent functional groups (sulfate, carboxyl, hydroxyl, hydroxymethyl, acetamido, etc.) and intersaccharide linkages to the higher energy transition of oxygen lone-pair electrons, giving basic information for understanding the detailed structures of GAGs in solution and for their theoretical assignment.
- 社団法人 日本農芸化学会の論文
- 2009-03-23
著者
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月向 邦彦
広島大学大学院理学研究科
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NAMATAME Hirofumi
Hiroshima Synchrotron Radiation Center, Hiroshima University
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TANIGUCHI Masaki
Hiroshima Synchrotron Radiation Center, Hiroshima University
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GEKKO Kunihiko
Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University
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Namatame Hirofumi
Department Of Physics University Of Tokyo
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Namatame Hirofumi
Department Of Physics Faculty Of Science University Of Tokyo
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Namatame Hirofumi
Hiroshima Synchrotron Radiation Center Hiroshima Univ.
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Namatame Hirofumi
Hiroshima Synchrotron Radiation Center Hiroshima Univ. Higashi-hiroshima 739-0046 Jpn
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Namatame H.
Hsrc Hiroshima Univ.
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Matsuo Koichi
Hiroshima Synchrotron Radiation Center, Hiroshima University
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Matsui T
Faculty Of Engineering University Of Miyazaki
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Gekko Kunihiko
Dep. Of Mathematical And Life Sciences Graduate School Of Sci. Hiroshima Univ.
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Gekko K
Department Of Mathematical And Life Sciences Graduate School Of Science Hiroshima University
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Gekko Kunihiko
The Department Of Mathematical And Life Sciences Graduate School Of Science Hiroshima University
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Gekko Kunihiko
Graduate School Of Science Hiroshima University:hiroshima Synchrotron Radiation Center Hiroshima Uni
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Gekko Kunihiko
Department Of Food Science And Technology Faculty Of Agriculture Nagoya University
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Namatame Hirofumi
Department Of Materials Science Facalty Of Science Hiroshima University
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Matsuo Koichi
Hiroshima Synchrotron Radiation Center Hiroshima University
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Gekko Kunihiko
Hiroshima University
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Taniguchi Masaki
Hiroshima Synchrotron Radiation Center Hiroshima University
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Namatame Hirofumi
Hiroshima Synchrotron Radiation Center (HSRC), Hiroshima University, Higashihiroshima, Hiroshima 739-0046, Japan
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