Mass Spectrometric Analysis Using Ruthenium (II)-Labeling for Identification of Glycosyl Hydrolase Product
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概要
- 論文の詳細を見る
Analysis of products digested by glycosyl hydrolases helps understanding of the hydrolysis mechanism and the substrate recognition in the enzymes. We developed a new universal technique, which consists of ruthenium (II) complex labeling and mass spectrometry analysis, to identify the reducing sugars released from oligosaccharides by enzymatic digestion. This method was applied to enzymatic digestion by chitinase and cellulase of the hyperthermophilic archaea Pyrococcus fusiosus and Pyrococcus horikoshii respectively.
- 社団法人 日本農芸化学会の論文
- 2009-02-23
著者
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YAMAMOTO Hitoshi
Department for the Administration of Safety and Hygiene (DASH), Osaka University
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OKAMURA Taka-aki
Department of Macromolecular Science, Graduate School of Science, Osaka University
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Ito Akihiro
東北大学 医学系研究科泌尿器科学
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Ito Akihiro
Department Of Urology Tohoku University Graduate School Of Medicine
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UEYAMA Norikazu
Department of Macromolecular Science, Graduate School of Science, Osaka University
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UEGAKI Koichi
National Institute of Advanced Industrial Science and Technology Kansai
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Yamamoto Hitoshi
Department Of Macromolecular Science Graduate School Of Science Osaka University
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Ishikawa Kazuhiko
National Institute of Bioscience and Human-Technology
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Kim Han-woo
National Inst. Of Advanced Industrial Sci. And Technol.
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NAKAMURA Tsutomu
National Institute of Advanced Industrial Science and Technology (AIST)
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Ueyama N
Department Of Macromolecular Science Graduate School Of Science Osaka University
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Ito Akihiro
Electric Power Research And Development Center Chubu Electric Power Co. Inc.
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Ishikawa Kazuhiko
National Inst. Advanced Industrial Sci. And Technol. Osaka Jpn
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Ueyama Norikazu
Department Oc Macromolecular Science Faculty Of Science Osaka University
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Uegaki Koichi
National Institute Of Advanced Industrial Science And Technology
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Kim Han-woo
National Institute Of Advanced Industrial Science And Technology
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Ishikawa Kazuhiko
National Institute Of Advanced Industrial Science And Technology
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Nakamura Tsutomu
National Inst. Advanced Industrial Sci. And Technol. Osaka Jpn
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ITO Akihiro
National Institute of Advanced Industrial Science and Technology
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Okamura Taka-aki
Department Of Macromolecular Science Graduate School Of Science Osaka University
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Okamura Taka-aki
Dept. Of Macromolecular Science Graduated School Of Science Osaka University
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Ishikawa Kazuhiko
National Chemical Laboratory For Industry
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Uegaki Koichi
National Inst. Of Advanced Industrial Sci. And Technol.
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Yamamoto Hitoshi
Department For The Administration Of Safety And Hygiene (dash) Osaka University
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Ito Akihiro
Departments of Urology, Tohoku University, Graduate School of Medicine
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