Denaturing Gradient Gel Electrophoresis Analysis of Lactic Acid Bacteria and Yeasts in Traditional Mongolian Fermented Milk

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概要

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• We analyzed lactic acid bacteria (LAB) and yeast populations in samples of airag, undaa, and hoormog, which are varieties of traditional fermented milk produced in Mongolia. To effectively detect lactobacilli, lactococci/streptococci/enterococci, and leuconostocs for complete analysis of the complex LAB population in airag, we developed two new primer sets and examined these primer sets in three different combinations using denaturing gradient gel electrophoresis (DGGE). DGGE analysis revealed the diversity of the LAB and yeast populations in these traditional fermented milk samples; six to 11 LAB and yeast species were detected. The species common to all samples include Lactobacillus helveticus, Leuconostoc mesenteroides, Lactococcus lactis, and Kluyveromyces marxianus. Lc. raffinolactis, Enterococcus faecium/ hirae/durans/villorum/ratti, Streptococcus thermophilus, L. kefiranofaciens, L. kefiri/buchneri, Leu. citreum, and Leu. lactis were detected in multiple samples, while lactose non-fermenting Saccharomyces cerevisiae was only detected in one sample of hoormog.

• 社団法人　日本食品科学工学会の論文
• 2010-07-01

著者

• Seto Yasuyuki

  Technology And Research Institute Snow Brand Milk Products Co. Ltd.

• Miyamoto Mari

  Technology And Research Institute Snow Brand Milk Products Co. Ltd.

• Miyamoto Taku

  Animal Food Function Graduate School Of Natural Science And Technology Okayama University

• Nakajima Hadjime

  Technology And Research Institute Snow Brand Milk Products Co. Ltd.

• BURENJARGAL Sedkhuu

  Department of Veterinary Science and Biotechnology, Mongolian State University of Agriculture

• GOMBOJAV Altangerel

  Department of International Affairs, Mongolian State University of Agriculture

• DEMBEREL Shirchin

  Department of Veterinary Science and Biotechnology, Mongolian State University of Agriculture

• Gombojav Altangerel

  Department Of International Affairs Mongolian State University Of Agriculture

• Burenjargal Sedkhuu

  Department Of Veterinary Science And Biotechnology Mongolian State University Of Agriculture

• Demberel Shirchin

  Department Of Veterinary Science And Biotechnology Mongolian State University Of Agriculture

• SETO Yasuyuki

  Technology and Research Institute, Snow Brand Milk Products Co. Ltd.

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• Denaturing Gradient Gel Electrophoresis Analysis of Lactic Acid Bacteria and Yeasts in Traditional Mongolian Fermented Milk
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