国際宇宙ステーションにおける微生物モニタリングのための拭き取り法の検討

概要

論文の詳細を見る
In closed ecological life support systems such as the International Space Station (ISS), the atmosphere and water are continuously recirculated, and the risk of infection increases when bacteria survive and proliferate. Microbial monitoring is important to assure the health and safety of staffs who live in these environments for extended periods. Swab method is currently used for microbial monitoring in the ISS, but the inter-operator variation is high because the protocol is not standardized and staff members without microbiological background must perform the sampling procedures. Here, we describe a simple swab procedure with higher operability and fewer individual differences than current methods. Concretely, we adopted the technique to swab one-way horizontally and vertically on the surface every cm without changing the contact face of the swab. Culture-independent fluorescence microscopy was used to count microbial cells for more accurate monitoring. Our protocol is simple and reliable, and it should be useful for microbial monitoring in the ISS.
生態工学会の論文
2010-01-31