An insertion of intracisternal A-particle retrotransposon in a novel member of the phosphoglycerate mutase family in the lew allele of mutant mice

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概要

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• Intracisternal A-particle retrotransposons (IAPs) are known, moveable, retrovirus-like elements and are defective in envelope protein synthesis in the mouse genome. Insertion of IAP elements can either interupt or enhance gene function or expression. Using a mouse model called lethal wasting (lew), we recently identified the insertion of an IAP sequence in a gene, 9630033F20Rik, that contains domains involved in glycolysis. The expression pattern of the 9630033F20Rik gene between various normal and diseased tissues was determined by semi-quantitative RT-PCR. The effect of the insertion mutation in 9630033F20Rik on glycolysis in heart, muscle, and brain tissues was further investigated using oligonuleotide microarray analysis. Results indicated that the expression of 9630033F20Rik is ubiquitous and its signal is relatively higher in heart and brain tissues. The insertion caused the deletion of exon 5 and decreased expression of this gene in all the tissues studied in the lew mice. Changes in the expression levels of glycolytic genes mainly occured in muscle tissue, raising a possibility that 9630033F20Rik may function as one of the transcriptional regulators of glycolytic genes in skeletal muscle. However, considering the fact that a single nucleotide mutation in vesicle-associated membrane protein 1 (VAMP1) has been reported as the causal gene for the lew mouse, how much of an impact the IAP insertion in the lew mouse phenotype has on glycolytic genes compared to the effect from the VAMP1 mutation responsible for the lew mouse phenotype should be further investigated.

• 2009-10-25

著者

• JIAO Yan

  Departments of Orthopaedic Surgery-Campbell Clinic and Pathology, University of Tennessee Health Sci

• YAN Jian

  Departments of Orthopaedic Surgery-Campbell Clinic and Pathology, University of Tennessee Health Sci

• GU Weikuan

  Departments of Orthopaedic Surgery-Campbell Clinic and Pathology, University of Tennessee Health Sci

• Jiao Yan

  Departments Of Orthopaedic Surgery-campbell Clinic And Pathology University Of Tennessee Health Scie

• Li Xinmin

  Ucla Clinical Microarray Core Department Of Pathology & Laboratory Medicine University Of Califo

• Gu Weikuan

  Departments Of Orthopaedic Surgery-campbell Clinic And Pathology University Of Tennessee Health Scie

• JIN Xiudong

  Mudanjiang Medical College

• JIAO Feng

  Departments of Orthopaedic Surgery- Campbell Clinic and Pathology, University of Tennessee Health Sc

• LI Xinmin

  Clinical Microarray Core, Department of Pathology & Laboratory Medicine, University of California at

• ROE Bruce

  Department of Chemistry and Biochemistry, University of Oklahoma

• JARRETT Harry

  Department of Chemistry, University of Texas at San Antonio

• Jiao Feng

  Departments Of Orthopaedic Surgery- Campbell Clinic And Pathology University Of Tennessee Health Sci

• Roe Bruce

  Department Of Chemistry And Biochemistry University Of Oklahoma

• Jarrett Harry

  Department Of Chemistry University Of Texas At San Antonio

• Jarrett Harry

  Department Of Biochemistry College Of Medicine University Of Tennessee

• Gu Weikuan

  Department Of Orthopaedic Surgery-campbell Clinic And Pathology University Of Tennessee Health Science Center (uthsc)

• Jiao Yan

  Department Of Medicine University Of Tennessee Health Science Center

• Cao Yanhong

  Institute Of Kaschin-beck Disease Center For Endemic Disease Control Centers For Disease Control And Prevention Harbin Medical University

• Jiao Feng

  Department Of Orthopaedic Surgery-campbell Clinic And Pathology University Of Tennessee Health Science Center (uthsc)

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