Heterocyclic Organobismuth(III) Compound Targets Tubulin to Induce G_2/M Arrest in HeLa Cells

スポンサーリンク

概要

• 論文の詳細を見る

• Our previous study showed that organobismuth compounds induce apoptosis in human promyelocytic leukemia cells, although solid tumor cell lines were relatively resistant. Herein, we investigated the primary cellular target of these compounds in HeLa cells. One organobismuth compound, bi-chlorodibenzo[c,f][1,5]thiabismocine (compound 3), arrested the cell cycle at G2/M as assessed by flow cytometry and by upregulating the expression of cyclin B1. At a low concentration (0.5 μM), compound 3 caused cell cycle arrest at the mitotic phase and induced apoptosis. At a higher concentration (>1.0 μM), it induced an arrest in the G2/M phase, leading to apoptosis. In many cells blocked at the M phase, the organization of microtubules was affected, indicating depolymerization of the microtubule network. Western blotting demonstrated that compound 3 depolymerized microtubules similar to colchicine and nocodazole. Experiments in vitro also showed that compound 3 inhibited the assembly of purified tubulin in a concentration-dependent manner by interacting with the colchicine-binding site of tubulin through its SH groups. Heterocyclic organobismuth compounds are novel tubulin ligands.

• 2009-04-20

著者

• Akagi Kiwamu

  Division Of Molecular Diagnosis And Cancer Prevention Saitama Cancer Center

• YAGURA Tatsuo

  Department of Bioscience, Faculty of Science and Technology, Kwansei-Gakuin University

• Yagura Tatsuo

  Department Of Biology Faculty Of Science Hokkaido University:(present)department Of Immunology And V

• IUCHI Katsuya

  Department of Bioscience, Faculty of Science and Technology, Kwansei Gakuin University

• Iuchi Katsuya

  Department Of Bioscience Faculty Of Science And Technology Kwansei Gakuin University

関連論文

• Heterocyclic Organobismuth(III) Compound Targets Tubulin to Induce G_2/M Arrest in HeLa Cells
• Leptin Inhibits Food Intake without Affecting Brain NOx Levels in Food-Deprived Mice(Pharmacology)
• Synchronous Alterations of RNA Synthesis and DNA-dependent RNA Polymerase Activities during Development of Dictyostelium discoideum
• Assembly of Envelope Structure with Vesicles Associated with Ku-Homologous Protein in Xenopus Egg Extract in the Absence Chromatin
• Expression of Golgi Membrane Protein p138 is Cell Cycle-independent and Dissociated from Centrosome Duplication
• EXPRESSION OF THE XENOPUS HOMOLOGUES OF HUMAN UBIQUITIN-CONJUGATING ENZYME E2 IN THE OOCYTE MATURSATION PROCESS OF XENOPUS(Developmental Biology,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan)
• Novel Monoclonal Antibody mAb G3A5 Recognizes 138-kDa Glyocoprotein Localized on the Golgi Membrane

スポンサーリンク