Agrobacterium-mediated transformation of the wild orchid species Phalaenopsis amabilis
スポンサーリンク
概要
- 論文の詳細を見る
Phalaenopsis hybrids constitute a major ornamental crop. An important parent species for many of these hybrids is Phalaenopsis amabilis. We developed a convenient method for the genetic modification of P. amabilis using Agrobacterium tumefaciens. The transformed intact protocorms, which are young orchid seedlings of P. amabilis, regenerated plants under the same conditions that showed the highest frequency of shooting. A kanamycin resistance gene under the control of the 35S promoter can be used as a selective marker. In addition, T-DNA vectors containing the Arabidopsis class 1 KNOX gene, BP/KNAT1, were successfully introduced into protocorms. Shoots were generated with an abnormal leaf shape that was easily distinguished from that of normal shoots, indicating that BP/KNAT1 can be used as a visible marker gene. Furthermore, the protocorms transformed with BP/KNAT1 produced multiple shoots. Both the presence and expression of the transgene in transformed plants were confirmed by molecular analysis.
- 日本植物細胞分子生物学会の論文
- 2007-06-01
著者
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MACHIDA Yasunori
Division of Biological Science, Graduate School of Science, Nagoya University
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Yoshioka Yasushi
Division of Biological Science, Graduate School of Science, Nagoya University
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Machida Chiyoko
Plant Biology Research Center, Chubu University
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Machida Y
Nagoya Univ. Nagoya Jpn
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Machida Yasunori
Division Of Biological Science Graduate School Of Science Nagoya University
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Semiarti Endang
Faculty Of Biology Gadjah Mada University
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Machida C
College Of Bioscience And Biotechnology Chubu University
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Yoshioka Yasushi
Division Of Biological Science Graduate School Of Science Nagoya University
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INDRIANTO Ari
Faculty of Biology, Gadjah Mada University
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PURWANTORO Azis
Faculty of Agriculture, Gadjah Mada University
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ISMININGSIH Sulastri
Center Study of Biotechnology, Gadjah Mada University
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SUSENO Nilo
Faculty of Biology, Gadjah Mada University
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ISHIKAWA Takaaki
Division of Biological Science, Graduate School of Science, Nagoya University
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Purwantoro Azis
Faculty Of Agriculture Gadjah Mada University
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Isminingsih Sulastri
Center Study Of Biotechnology Gadjah Mada University
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Suseno Nilo
Faculty Of Biology Gadjah Mada University
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Indrianto Ari
Faculty Of Biology Gadjah Mada University
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Machida Chiyoko
Divison Of Biological Science Graduate School Of Science Nagoya University
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Ishikawa Takaaki
Division Of Biological Science Graduate School Of Science Nagoya University
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Machida Chiyoko
Div. Biol. Sci. Grad. Sch. Sci. Nagoya Univ.
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