Collagen-Carboxymethyl Dextran Conjugate without Platelet Aggregation

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概要

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• Pepsin solubilized collagen-carboxymethyl dextran (PSC-CMD) conjugate was prepared to improve the function of collagen by using cyanogen bromide. The conjugation between PSC and CMD was confirmed by SDS-PAGE, isoelcctric focusing and size-exclusion chromatography. The molar ratio of the conjugate was PSC:CMD=1:2.3. The isoelectric point was shifted from 9.0 to 5.5 by conjugation. Denaturation temperature of the conjugate molecule was 42.2°C, which is 1.9°C higher than that of PSC. The PSC-CMD conjugate maintained self-assembling ability but showed a very slow aggregation as compared with that of PSC. Reconstructed matrix of the conjugate had lower denaturation temperature and random molecular rearrangement structure as compared with PSC matrix. The platelet aggregation inducing ability, analyzed by spectroscopy disappeared.

• 社団法人 日本畜産学会の論文
• 1999-07-25

著者

• HATTORI Makoto

  Astronomical Institute, Tohoku University

• Hattori M

  Faculty Of Agriculture Tokyo University Of Agriculture And Technology

• TAKAHASHI Koji

  Faculty of Agriculture, Tokyo Noko University

• Hattori M

  Astronomical Institute Tohoku University

• Takahashi K

  Faculty Of Agriculture Tohoku University

• Toyokawa Tetsuya

  Faculty Of Agriculture Tokyo University Of Agriculture And Technology

• HATTORI Makoto

  Faculty of Agriculture, Tokyo University of Agriculture and Technology

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