Identification of three Asian Hediste species (Polychaeta : Nereididae) by PCR-RFLP analysis of the mitochondrial 16S rRNA gene
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概要
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An easy polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis was designed to identify the three morphologically similar nereidid polychaete species Hediste atoka, Hediste diadroma and Hediste japonica which often dominate in estuarine macrobenthic fauna in eastern Asia. Restriction digestion analysis of the PCR products of the partial mitochondrial 16S rRNA gene (approximately 600 bp) of these three species, using EcoRV and TspEI endonucleases, generated species-specific restriction patterns. The PCR-RFLP method enables easy and accurate identification of the three Hediste species.
- 日本プランクトン学会、日本ベントス学会の論文
- 2008-02-01
著者
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Sato Masanori
Department of Earth and Environmental Sciences, Faculty of Science, Kagoshima University
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Tosuji Hiroaki
Department Of Biology Faculty Of Science Toho University
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Tosuji Hiroaki
Department Of Chemistry And Bioscience Faculty Of Science Kagoshima University
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Sato Masanori
Dep. Of Earth And Environmental Sciences Graduate School Of Sci. And Engineering Kagoshima Univ.
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Sato Masanori
Department Of Earth And Environmental Sciences Faculty Of Science Kagoshima University
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Sato Masanori
Department Of Chemical Engineering Faculty Of Engineering Kyushu University
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Sato Masanori
Department Of Applied Physics Tokyo University Of Agriculture And Technology
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