Manipulation of Fish Germ Cell : Visualization, Cryopreservation and Transplantation
スポンサーリンク
概要
- 論文の詳細を見る
Germ-cell transplantation has many applications in biology and animal husbandry, including investigating the complex processes of germ-cell development and differentiation, producing transgenic animals by genetically modifying germline cells, and creating broodstock systems in which a target species can be produced from a surrogate parent. The germ-cell transplantation technique was initially established in chickens using primordial germ cells (PGCs), and was subsequently extended to mice using spermatogonial stem cells. Recently, we developed the first germ-cell transplantation system in lower vertebrates using fish PGCs and spermatogonia. During mammalian germ-cell transplantation, donor spermatogonial stem cells are introduced into the seminiferous tubules of the recipient testes. By contrast, in the fish germ-cell transplantation system, donor cells are microinjected into the peritoneal cavities of newly hatched embryos; this allows the donor germ cells to migrate towards, and subsequently colonize, the recipient genital ridges. The recipient embryos have immature immune systems, so the donor germ cells can survive and even differentiate into mature gametes in their allogeneic gonads, ultimately leading to the production of normal offspring. In addition, implanted spermatogonia can successfully differentiate into sperm and eggs, respectively, in male and female recipients. The results of transplantation studies in fish are improving our understanding of the development of germ-cell systems during vertebrate evolution.
- 2006-12-01
著者
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KOBAYASHI Terumasa
Department of Marine Biosciences, Tokyo University of Marine Science and Technology
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YOSHIZAKI Goro
Department of Marine Biosciences, Tokyo University of Marine Science and Technology
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Takeuchi Y
Research Center For Advanced Science And Technology Tokyo University Of Marine Science And Technolog
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Takeuchi Yutaka
Electrotechical Laboratory Tsukuba Science City
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Nagasawa Kazue
Department Of Marine Biosciences Tokyo University Of Marine Science And Technology
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Yoshizaki G
Department Of Marine Biosciences Tokyo University Of Marine Science And Technology
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Yoshizaki Goro
Department Of Aquatic Biolsciences Tokyo University Of Fisheries
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Kobayashi T
Kinki Univ. Nara Jpn
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OKUTSU Tomoyuki
Department of Marine Biosciences, Tokyo University of Marine Science and Technology
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YANO Ayaka
Department of Marine Biosciences, Tokyo University of Marine Science and Technology
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SHIKINA Shinya
Department of Marine Biosciences, Tokyo University of Marine Science and Technology
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TAKEUCHI Yutaka
Department of Marine Biosciences, Tokyo University of Marine Science and Technology
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Yano Ayaka
Department Of Marine Biosciences Tokyo University Of Marine Science And Technology
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Shikina Shinya
Department Of Marine Biosciences Tokyo University Of Marine Science And Technology
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Okutsu Tomoyuki
Department Of Marine Biosciences Tokyo University Of Marine Science And Technology
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Kobayashi Terumasa
Department Of Marine Biosciences Tokyo University Of Marine Science And Technology
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Takeuchi Yutaka
Department Of Aquatic Bio-sciences Tokyo University Of Fisheries
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