High-Resolution Ultrasonography of Xenografted Domestic Cat Ovarian Cortex

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概要

• 論文の詳細を見る

• Transplantation of ovarian tissue has high potential for female gamete conservation. However, optimal timing of oocyte recovery for in vitro maturation and fertilization is still critical. Therefore the aim of the present study was to use high-resolution transcutaneous ultrasonography to monitore follicular development within xenografted ovarian tissue. Ovarian cortex fragments (n=44) from domestic cats were transplanted into athymic nude rats (n=12). Graft development in the animals was assessed weekly by high frequency ultrasound (10-22 MHz) under two different FSH regimes. Blood collection for serum estradiol determination and vaginal smears were performed simultaneously. The xenografts were removed at different time points according to the ultrasound findings. The survival rate of the transplants 4 weeks after surgery was 54.5% and antral follicular growth was observed within 10 grafts from 5 different hosts (8.6 ± 6.43 follicles per graft). Early follicle antrums could be detected from 0.4 mm onwards. The growth rate of the antral cavity was calculated from weekly measurements (0.56 ± 0.44 mm per week). Although vaginal cells and estradiol levels followed a cyclic pattern, no correlation was found between follicular diameter, estradiol and keratinized vaginal cells. We recovered 5, 1 and 4 cumulus oocyte complexes from three different individuals during weeks 19, 21, and 23 respectively. Extrusion of a polar body (1 oocyte) and germinal vesicle break down (7 oocytes) indicated progression of maturation after in vitro culture. We conclude that ultrasonography und provided a reliable method to examine xenograft survival and follicular development within the grafts. Furthermore, this technique is suitable for assessment of the efficiency of hormonal treatment and narrowing of the optimal time frame for oocyte retrieval. To our knowledge, this is the first report of the in vivo development of early antral follicles in mammalian species.

• 日本繁殖生物学会の論文
• 2007-10-01

著者

• Fassbender Mirja

  Leibniz Institute For Zoo And Wildlife Research

• Hildebrandt Thomas

  Leibniz Institute For Zoo And Wildlife Research

• PARIS Monica

  Institute Biomedical & Life Sciences, West Medical Building, University of Glasgow

• COLENBRANDER Ben

  Department of Farm Animal Health, University of Utrecht

• JEWGENOW Katarina

  Leibniz Institute for Zoo and Wildlife Research

• Paris Monica

  Institute Biomedical & Life Sciences West Medical Building University Of Glasgow

• Colenbrander Ben

  Department Of Farm Animal Health University Of Utrecht

関連論文

• High-Resolution Ultrasonography of Xenografted Domestic Cat Ovarian Cortex
• The Secretory Pattern and Source of Immunoreactive Prolactin in Pregnant African (Loxodonta africana) and Asian (Elephas maximus) Elephants
• The Secretory Pattern and Source of Immunoreactive Prolactin in Pregnant African (Loxodonta africana) and Asian (Elephas maximus) Elephants

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