Determination of a complete lemur mitochondrial genome from feces

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概要

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• Mitochondrial genome (mt-genome) sequences are widely used in molecular phylogenetic studies because of their relatively small size and of the strict orthology of their genes. It is sometimes the most difficult step in such studies to get samples particularly from endangered species. Here, we report successful amplification and sequencing of mt-genome of Propithecus (sifaka) from feces sample, using the extract method of Chelex-100 Phenol-Chloroform or QIAamp DNA Stool kit (Qiagen) in combination with FTA cards (Whatmann). For biologists, such a noninvasive sampling method should be an important resource that will provide greater opportunities to collect invaluable samples. By using the mt-genome sequence of sifaka obtained by this work with other published sequences of primates, we estimated the phylogeny of primates, and demonstrated that the evolutionary rate acceleration occurred in the Anthropoids lineage after they diverged from tarsier.

• 日本哺乳類学会の論文
• 2007-06-01

著者

• MATSUI Atsushi

  Department of Clinical Oncology, The Institute of Medical Science, The University of Tokyo, Departme

• Matsui Atsushi

  Department Of Biosystems Science The Graduate University For Advanced Studies (sokendai)

• HASEGAWA Masami

  Department of Oral Microbiology, School of Dentistry

• Hasegawa Masami

  Department Of Biology Faculty Of Science Toho University

• HORAI Satoshi

  Department of Biosystems Science, The Graduate University for Advanced Studies (Sokendai)

• RAKOTONDRAPARANY Felix

  Department of Animal Zoology, University of Antananarivo

• Horai Satoshi

  Department Of Biosystems Science The Graduate University For Advanced Studies

• Rakotondraparany Felix

  Department Of Animal Zoology University Of Antananarivo

• Hasegawa Masami

  Department Of Biosystems Science The Graduate University For Advanced Studies (sokendai)

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