神経病原性レトロウイルスA8-Vの env 遺伝子発現に見られた奇妙なスプライシング

概要

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原著Packaging cell lines PacNIH/A8β and PacNIH/A8δ, which were introduced by the DNA of expression vectors pA8(Ψ-)βand pA8(Ψ-)δ ｒe spective1y, to produce pseud-retroviral particles in the retroviral vector system, were established. Both the expression vectors were constructed using the genes of A8-V, which is an isolate from Friend murine leukemia virus(F-MLV) and shows higher proliferation rates in the central nervous system(CNS) of rats and mice compared with original F-MLV. These two expression vectors utilize a different polyadenylation signal (polyA signal) for the expression of retroviral genes. The former employs a 0.85kb fragment in the SV40 early region as a polyA signal, and the latter, a 0.13kb of the sameregion. PacNIH/A8β showed an unstable expression of the env gene in the A8-V genome, whereas all of the retroviral gene expressions in the PacNIH/A8δ were perfect. An occurrence of abnormal splicing in the mRNA for the env gene in the PacNIH/A8β cells was suggested.
慶應医学会の論文
2006-03-25