A Mutant Mouse with Severe Anemia and Skin Abnormalities Controlled by a New Allele of the Flaky Skin (fsn) Locus

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概要

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• We found a novel recessive mutation in an inbred strain, INT, that was derived from an ICR closed colony. Mice homozygous for this mutation are identified by severe anemia, dysgenesis and neonatal death. This mutation was tentatively named int. Intercrosses of int heterozygotes (+/int) and the flaky skin heterozygotes (+/fsn) resulted in abnormal mice (int/fsn heterozygotes) showing anemia and flaky skin with the expected frequency for autosomal recessive mutation. The int gene was therefore named fsnJic as an allele of the fsn locus on chromosome 17. We carried out phenotype analyses using B6.INT- fsnJic mice to observe phenotypes of blood and skin in the embryonic and neonatal stages. Discrimination of fsnJic embryos from normal embryos was performed by an indirect diagnosis of the fsnJic gene using the D17Mit130 microsatellite marker tightly linked to the fsn locus. The number of fetal nucleated RBC of normal embryos decreased gradually to 17.5 dpc, but that of the abnormal embryos decreased to 14.5 dpc followed by a gradual increase to 17.5 dpc. Skin of fsnJic embryos did not show any abnormalities and expressed cytokeratins normally as skin epithelial cell markers at each embryonic stage (15.5 dpc to 18.5 dpc). Time differences in the appearance of the different phenotypes observed in various tissue and organs of fsn homozygotes suggest they are caused by expression of the fsn gene at different developmental stages.

• 社団法人　日本実験動物学会の論文

著者

• TAKABAYASHI Shuji

  Institute for Experimental Animals, Hamamatsu University School of Medicine

• Katoh Hideki

  Institute For Experimental Animals Hamamatsu University School Of Medicine

• Takabayashi Shuji

  Inst. For Experimental Animals Hamamatsu Univ. School Of Medicine

• Takabayashi Shuji

  Institute For Experimental Animals Hamamatsu University School Of Medicine

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