A Genetic Quality Testing System for Early Stage Embryos in the Mouse

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概要

• 論文の詳細を見る

• We have established a genetic quality testing system for early stage embryos of the mouse. A method of preparation of template DNA for PCR was established using the lysis buffer (1 × PCR reaction buffer supplemented with proteinase K at a concentration of 40 μg/ml) developed by the authors. We demonstrated that two 8-cell embryos of an inbred strain provide sufficient volumes of template DNA for PCR to identify the strain of embryos using four microsatellite markers (D3Mit54, D5Mit18, D6Mit15 and D8Mit50) differentiating 13 inbred strains of mice. This system will be useful in embryo banks that have recently been established worldwide for demonstrating the genetic accuracy of a given strain prior to recovery of live animals.

• 社団法人　日本実験動物学会の論文
• 2003-10-01

著者

• Katoh Hideki

  Institute For Experimental Animals Hamamatsu University School Of Medicine

• Muguruma Kaori

  Genetics Division Iclas Monitoring Center Central Institute For Experimental Animals

• ODA Koji

  Tanabe R&D Co., Ltd.

• Oda Koji

  Tanabe R&d Co. Ltd.

• HIOKI Kyoji

  Genetics Division, ICLAS Monitoring Center, Central Institute for Experimental Animals

• Oda Koji

  Tanabe R&d Service Co. Ltd.

• KATOH Hideki

  Institute for Experimental Animals, Hamamatsu University School of Medicine

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• Microsatellite Genotyping for Genetic Quality Testing Using Sperm Cells in the Mouse
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