A Trial to Assess the Amount of Insulin Antibodies in Diabetic Patients by Surface Plasmon Resonance

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概要

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• Objective To measure the amount and affinity of insulin antibodies, we performed a trial to establish a new method for quantitative and qualitative analysis of these antibodies by using surface plasmon resonance (BIAcoreTM system).Methods Real-time detection of insulin antibody interaction and kinetic analysis were performed using the BIAcoreTM system.Patients or Materials Eight diabetic patients with insulin antibodies and whose fasting total immunoreactive insulin levels were more than 100 μU/ml were selected. The patients with and without recurrent hypoglycemia were classified into hypoglycemic episode-positive or hypoglycemic episode-negative groups, respectively. Seven diabetic patients without insulin antibodies were selected as controls.Results In the 8 patients, the concentration of insulin antibodies ranged from 2.91 to 16.3 μg/ml and insulin antibodies were not detected in the control group. The apparent KD (dissociation constant) and kd (the dissociation rate constant) values of the patients were much larger than those seen for the anti-human insulin monoclonal antibody. The KD values were significantly higher in the hypoglycemic episode-positive group than in the hypoglycemic episode-negative group (p<0.05). No significant differences in the concentration, the ka (the association rate constant) and the kd values were noted between the groups.Conclusion The data suggests that insulin antibodies of the patients have an apparently lower affinity status in sera as compared with that for the anti-human insulin monoclonal antibody, and dissociate easily from the immune-complex in the sera, especially in cases where there is recurrent hypoglycemia in the patients. Therefore insulin antibody characteristics are one of the causative factors in hypoglycemic episodes.

• 社団法人　日本内科学会の論文
• 2005-02-01

著者

• Noritake Masayuki

  Department Of Cardiology Saitama University Medical Center

• Noritake Masayuki

  Department Of Internal Medicine 5 Tokyo Medical University

• WATANABE Toshiya

  Department of Technology Education, Shizuoka University

• NISHIGORI Hideo

  Faculty of Pharmaceutical Sciences, Teikyo University

• MATSUOKA Takeshi

  Department of Internal Medicine 5, Tokyo Medical Unvierstiy

• KURE Masahiko

  Department of Internal Medicine, Tokyo Medical University (Kasumigaura Hospital)

• KATSURA Yoshiya

  Department of Internal Medicine, Tokyo Medical University (Kasumigaura Hospital)

• KOSANO Hiroshi

  Faculty of Pharmaceutical Sciences, Teikyo University

• IWAKI Yoshiki

  Department of Internal Medicine, Tokyo Medical University (Kasumigaura Hospital)

• Matsuoka Takeshi

  Department Of Applied Physics Faculty Of Engineering Hokkaido University:(present Address)communicat

• Watanabe Toshiya

  Department Of Internal Medicine 5 Tokyo Medical University

• Watanabe Toshiya

  Department Of Cardiology National Nagoya Hospital

• Kure Masahiko

  Department Of Internal Medicine 5 Tokyo Medical University

• Iwaki Yoshiki

  Department Of Internal Medicine 5 Tokyo Medical University

• Kosano Hiroshi

  Faculty Of Pharmaceutical Sciences Teikyo University

• Matsuoka Takeshi

  Department Of Internal Medicine 5 Tokyo Medical University

• Katsura Yoshiya

  Department Of Internal Medicine 5 Tokyo Medical University

• Nishigori Hideo

  Faculty Of Pharmaceutical Sciences Teikyo University

• Watanabe Toshiya

  Department Of Biochemistry Osaka University Medical School

• NISHIGORI Hideo

  Faculty of Pharmaceutical Science, Teikyo Univeristy

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