Compositions of Anthocyanin and Other Flavonoids in Cultured Cells of Rabbiteye Blueberry (Vaccinium ashei Reade cv. Tifblue)

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概要

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• Our blueberry cultured cells produce anthocyanins in high quantity (Nawa et al., Biosci. Biotech. Biochem., 57, 770-774, 1993). Using high performance liquid chromatography/mass spectrometry (LC-MS), 14 anthocyanins in the red cells derived from leaves of rabbiteye blueberry cv. Tifblue (Vaccinium ashei Reade) were identified as 3-galactoside (gal), 3-glucoside (glc) and 3-arabinoside (ara) of cyanidin (Cy), delphinidin (Dp), petunidin (Pe), peonidin (Pn) and malvidin (Mv), except for Mv 3-ara. The fruits additionally contained 15 anthocyanins and Mv 3-ara, as a main anthocyanin. The autumn red leaves contained Cy 3-gal, Cy 3-ara and Cy 3-glc. In the red cells and the leaves, derivatives of Cy were the most abundant at 85.7% (Cy 3-gal 49.1%, Cy 3-glc 5.8%, Cy 3-ara 30.9%) and 100% (Cy 3-gal 51.0%, Cy 3-glc 6.5%, Cy 3-ara 42.5%) of the total anthocyanins, respectively. In contrast, the fruits contained Mv derivatives as main components that amounted to 50.9% of the total anthocyanins; Mv 3-gal, Mv 3-ara and Mv 3-glc constituted 21.2, 15.3 and 14.4%, respectively. Cy derivatives in fruit anthocyanins amounted to 18.0%. The red cells produced 107 mg of anthocyanin/100 g fr wt, which was slightly lower than that of the fruits (129 mg). LC-MS analysis also suggested the presence of quercetin (Q) 3-glc, Q 3-rutinoside, and glycosides of myricetin (M) and Q in the red cells. This analysis showed the presence of (+)-catechin (C) and (−)-epicatechin (EC) in these cells as well as of (+)- C, (−)-EC and chlorogenic acid in the red leaves. These flavonoids and phenolics were also some of the main components in the leaves.

• 2004-08-01

著者

• Nawa Yoshihiko

  National Food Research Institute

• Hamamatsu Shioka

  National Food Research Institute

• YABE Kimiko

  National Institute of Animal Health

• Yabe Kimiko

  National Food Research Institute

関連論文

• Production of Anthocyanins, Carotenoids, and Proanthocyanidins by Cultured Cells of Rabbiteye Blueberry (Vaccinium ashei Reade)
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