Identification of Amino Acid Residues Essential for the Catalytic Reaction of Bacillus kaustophilus Leucine Aminopeptidase

スポンサーリンク

概要

• 論文の詳細を見る

• The functional significance of amino acid residues Lys-265, Asp-270, Lys-277, Asp-288, Asp-347, Glu-349, and Arg-351 of Bacillus kaustophilus leucine aminopeptidase was explored by site-directed mutagenesis. Variants with an apparent molecular mass of approximately 54 kDa were overexpressed in Escherichia coli and purified to homogeneity by nickel-chelate chromatography. The purified mutant enzymes had no LAP activity, implying that these residues are important for the catalytic reaction of the enzyme.

• 社団法人 日本農芸化学会の論文
• 2004-08-23

著者

• Lin Long-Liu

  Department of Applied Chemistry, National Chiayi University

• Lin L‐l

  Department Of Applied Chemistry National Chiayi University

• Chi M‐c

  Graduate Institute Of Biotechnology National Chiayi University

• Chi Meng-chun

  Graduate Institute Of Biotechnology National Chiayi University

• CHOU Wei-Mou

  Graduate Institute of Biotechnology, National Chiayi University

• HSU Wen-Hwei

  Institute of Molecular Biology, National Chung Hsing University

• Hsu Wen-hwei

  Institute Of Molecular Biology National Chung Hsing University

• Chou Wei-mou

  Graduate Institute Of Biotechnology National Chiayi University

• Lin Long-liu

  Department Of Applied Chemistry National Chia-yi University

関連論文

• Engineering of a truncated α-amylase of Bacillus sp. strain TS-23 for the simultaneous improvement of thermal and oxidative stabilities(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
• Identification of Amino Acid Residues Essential for the Catalytic Reaction of Bacillus kaustophilus Leucine Aminopeptidase
• Essential covalent linkage between the chymotrypsin-like domain and the extra domain of the SARS-CoV main protease
• Genome-Wide Transcript Expression Analysis in the Uterovaginal Junction in Association with Fertile Period in Tsaiya Ducks
• Genome-Wide Transcript Expression Analysis in the Uterovaginal Junction in Association with Fertile Period in Tsaiya Ducks

スポンサーリンク