Partial Genomic Sequences of S_6-, S_<12>-, S_<13>-, S_<14>-, S_<17>-, S_<19>-, and S_<21>-RNases of Apple and Their Allele Designations

概要

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We determined partial genomic sequences including a hypervariable HVa (RHV) region and introns of the S6-, S12-, S13-, S14-, S17-, S19-, and S21-RNases of apple. Previously, it was suggested by Broothaerts (2003) that the S6 corresponded to S25 in ‘McIntosh’/‘Wijcik’ and ‘Tydeman’s Early Worcester’, and S19 corresponded to S28. However, S6 and S19 are different from the S25 and S28, respectively. The determined sequences of the S6 and S12, and S17 and S19 are identical, and these four alleles seem to act as the same allele. We newly assigned S6a in place of S6 and S12, and S6b in place of S17 and S19 from the results of sequence analyses, not pollination tests. The sequences of S13 and S14 were also identical. We also newly assigned S11 in place of S13 and S14. We had speculated that S21 would correspond to St by PCR-digestion analysis, however, the determined sequence of S21 was different from St.
日本植物細胞分子生物学会の論文
2003-12-01