糖尿病ラット筋肉における酸化傷害タンパク質の検出

概要

論文の詳細を見る
Oxidative stress is implicated in a broad variety of chronic and acute diseases, including such age-related diseases as diabetes. To understand at the protein level cellular damage caused by the stress, we developed a proteomic method, in which protein carbonyls were derivatized with biotin hydrazide followed by two-dimensional gel electrophoresis with agarose gels in the first dimension. The method, being capable of analyzing high-molecular-mass proteins as large as myosin heavy chains (molecular mass: 200 kDa), was applied to detecting protein carbonyls in muscles of a diabetes model OLETF rat and a control LETO rat. A number of proteins, including mitochondrial ATP synthase β-chain, desmin, actin, and myosin, were found carbonylated. Our method would provide a means toward clarifying a comprehensive view of oxidative modifications of proteins during a long progression of age-related diseases and understanding the mechanism of the onset, progression, and complication of the diseases.
日本質量分析学会の論文
2003-10-01