PCR primers useful for nucleotide sequencing of rDNA of the powdery mildew fungi

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概要

• 論文の詳細を見る

• Four PCR primers that are useful to determine the nucleotide sequences of the rDNA of the powdery mildew fungi were newly designed. These primers provide both enough stability to work on a wide range of powdery mildews and enough specificity to eliminate contaminating DNA by PCR.DNA sequences of the rDNA ITS region were successfully obtained from specimens that were contaminated by other fungi. In addition, sequence results of the 18S and 28S rDNA were dramatically improved by using these primers in most of the specimens examined.

• Mycological Society of Japanの論文
• 2001-02-15

著者

• Kano Yukiko

  Laboratory Of Plant Pathology Faculty Of Bioresources Mie University

• Takamatsu Susumu

  Laboratory Of Plant Pathology Faculty Of Bioresources Mie University

関連論文

• Cloning and sequence analysis of the plasmid DNA found in Rhizoctonia solani AG-2-2 LP isolate and its potential use for fungal detection
• PCR primers useful for nucleotide sequencing of rDNA of the powdery mildew fungi
• Nucleotide sequence diversity of rDNA internal transcribed spacers extracted from conidia and cleistothecia of several powdery mildew fungi

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