Cloning and sequence analysis of the plasmid DNA found in Rhizoctonia solani AG-2-2 LP isolate and its potential use for fungal detection
スポンサーリンク
概要
- 論文の詳細を見る
A plasmid DNA (PE-42 plasmid) obtained from Rhizoctonia solani AG-2-2 LP isolate PE-42, the causal agent of large patch disease of zoysiagrass (Zoysia spp.), was partially cloned. Sequence analyses of the 1.2-kb and 0.2-kb cloned fragments revealed that the nucleotide sequence of the 0.2-kb fragment was similar to that of the 5' region of the 1.2-kb fragment (pSH4). Southern hybridization analysis of total DNA of a large patch isolate using the 1.2-kb fragment as a probe showed two bands differing slightly in size. These results indicated that the PE-42 plasmid consisted of at least two components having similar nucleotide sequences with different sizes. The nucleotide sequence of the pSH4 fragment showed no significant homology with known DNA sequences. The pSH4 fragment hybridized to all of the 22 large patch isolates tested, but not to other subgroup isolates in AG-2-2, other anastomosis groups of R. solani, or other pathogens of zoysiagrass. These results indicated that the pSH4 fragment can be used as a specific probe to detect the large patch fungus. The detection limit for the large patch fungus using the pSH4 fragment as a probe was <special>0.1 μg </special>of the total DNA of the fungus, which was significantly higher than those for other fungi. However, with improvement of the detection sensitivity and simplification of the detection procedure, the pSH4 fragment has potential for use in molecular diagnosis of the large patch disease of zoysiagrass.
- 1999-02-15
著者
-
Kunoh Hitoshi
Laboratory Of Plant Pathology Faculty Of Bioresources Mie University
-
Kunoh Hitoshi
Laboratory Of Ecological Circulation Mie University
-
Takamatsu Susumu
Laboratory Of Plant Pathology Faculty Of Bioresources Mie University
-
NAKANO Manami
Laboratory of Plant Pathology, Faculty of Bioresources, Mie University
-
KUNASAKDAKUL Kaewalin
Department of Plant Pathology, Faculty of Agriculture, Chiangmai University
-
YOKOTA Hideyuki
Laboratory of Plant Pathology, Faculty of Bioresources, Mie University
-
Kunasakdakul Kaewalin
Department Of Plant Pathology Faculty Of Agriculture Chiangmai University
-
Nakano Manami
Laboratory Of Plant Pathology Faculty Of Bioresources Mie University
-
Yokota Hideyuki
Laboratory Of Plant Pathology Faculty Of Bioresources Mie University
関連論文
- Actin Microfilaments are Required for the Expression of Nonhost Resistance in Higher Plants
- Fistupyrone, a Novel Inhibitor of the Infection of Chinese Cabbage by Alternaria brassicicola, from Streptomyces sp. TP-A0569
- Multiplication of isolate R-5 of Streptomyces galbus on rhododendron leaves and its production of cell wall-degrading enzymes
- Association of Induced Disease Resistance of Rhododendron Seedlings with Inoculation of Streptomyces sp. R-5 and Treatment with Actinomycin D and Amphotericin B to the Tissue-culture Medium
- Studies on Endophytic Actinomycetes (I) : Streptomyces sp. Isolated from Rhododendron and Its Antifungal Activity
- Inhibition of ATPase Activity in Pea Plasma Membranes In Situ by a Suppressor from a Pea Pathogen, Mycosphaerella pinodes
- Cloning and sequence analysis of the plasmid DNA found in Rhizoctonia solani AG-2-2 LP isolate and its potential use for fungal detection
- Studies on turfgrass snow mold caused by Typhula ishikariensis. II. Microscopical observation of infected bentgrass leaves
- Identification of endophytic Streptomyces sp. R-5 and analysis of its antimicrobial metabolites
- PCR primers useful for nucleotide sequencing of rDNA of the powdery mildew fungi
- Rapid pregermination and germination responses of Erysiphe pisi conidia to contact and light
- Nucleotide sequence diversity of rDNA internal transcribed spacers extracted from conidia and cleistothecia of several powdery mildew fungi
- An Endophytic Actinomycete,Sterptomyces sp.AOK-30,Isolated from Mountain Laurel and Its Antifungal Activity
- Elicitor Activity of a Fungal Product Assessed at the Single-Cell Level by a Novel Gel-Bead Method
- Release of xylanase from conidia and germlings of Blumeria graminis f. sp. tritici and expression of a xylanase gene
- Genes expressed in tissue-cultured seedlings of mountain laurel (Kalmia latifolia L.) with colonizing Streptomyces padanus AOK30