FRACTIONATION OF THE β-GLUCOSIDASE COMPONENT FROM CELLULASE BY AFFINITY PRECIPITATION AND PRODUCTION OF CELLOBIOSE BY THE RESIDUAL ENZYMES

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概要

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• For the purpose of cellobiose production from cellulose, removal of β-glucosidase from cellulase was studied. This purpose can be achieved by an affinity precipitation technique using a reversibly soluble polymer depending on PH.The polymer was hydroxypropyl methyl cellulose acetate succinate (AS-LF). β-glucosidase did not conjugate AS-LF, while the rest of the components did. The conjugated components gave high cellobiohydrolase and endo-β-1,4-glucanase activities. These components were detached by centrifuging with 1.0 M acetate buffer, pH 3.5 from AS-LF, but it was impossible to detach these components by 0.1 M acetate buffer, pH 5.0.Hydrolysis of three kinds (KC-flock, Pulp-flock and Avicel) of cellulose powders with these detached components from AS-LF was carried out. The soluble reducing sugar contained glucose and cellobiose, and the cellobiose content in the product was over 50 mol% for each substrate. Moreover, when the hydrolysis was carried out using the solubilized AS-LF conjugating the components of high cellobiohydrolase and endo-β-1,4-glucanase activities, continuous cellobiose production could be performed by means of repeated affinity precipitation as follows. The reaction products from the insolubilized AS-LF and the residual substrate were separated by adjusting the PH of the reaction medium at an appropriate interval, and the precipitates were dissolved again to continue the reaction.

• 社団法人 化学工学会の論文
• 1992-12-01

著者

• Kuroda Kenji

  Niigata University

• Taniguchi M

  Niigata Univ. Niigata Jpn

• HOMMA Taira

  Niigata University

• FUJII Michihiro

  Niigata University

• MORI Junichi

  Niigata University

• KAWAKAMI Tohru

  Niigata University

• TANIGUCHI Masayuki

  Niigata University

• Homma Taira

  Graduate School Of Natural Science And Technology Department Of Chemical Engineering Niigata Univers

• Fujii Michihiro

  Graduate School Of Natural Science And Technology Department Of Chemical Engineering Niigata Univers

• Fujii Michihiro

  Niigata College of Techno1gy University

関連論文

• FRACTIONATION OF THE β-GLUCOSIDASE COMPONENT FROM CELLULASE BY AFFINITY PRECIPITATION AND PRODUCTION OF CELLOBIOSE BY THE RESIDUAL ENZYMES
• FRACTIONATION OF THE β-GLUCOSIDASE COMPONENT FROM CELLULASE BY CELLULOSE COLUMNS AND PRODUCTION OF CELLOBIOSE BY THE RESIDUAL ENZYMES
• Continuous Simultaneous Saccharification and Fermentation of Delignified Rice Straw by a Combination of Two Reversibly Soluble-Autoprecipitating Enzymes and Pentose-Fermenting Yeast Cells.

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