Effect of Saiboku-to, an Antiasthmatic Herbal Medicine, on Nitric Oxide Generation from Cultured Canine Airway Epithelial Cells

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概要

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• The effect of <I>Saiboku-to</I> (TJ-96), an antiasthmatic Kampo medicine, on the generation of nitric oxide (NO) from cultured canine tracheal epithelium was investigated using a highly specific amperometric sensor for this molecule in vitro. Immersion of the NO-selective electrode in the medium containing tracheal epithelial cells detected the baseline current of 16.8 - 57.0 pA, which corresponded to an NO concentration ([NO]) of 39.7 ± 8.1 nM. Addition of TJ-96 increased [NO] in a concentration-dependent manner, the maximal increase from the baseline level and the concentration of TJ-96 required to produce a half-maximal effect (EC<SUB>50</SUB>) being 127.5 ± 20.1 nM (P < 0.001) and 86 ± 9 μg/ml, respectively. Pretreatment of cells with N<SUP>G</SUP>-nitro-L-arginine methylester (L-NAME) greatly inhibited the TJ-96-induced increase in [NO], whereas N<SUP>G</SUP>-nitro-D-arginine methylester (D-NAME) had no effect, and this inhibition was reversed by L-arginine but not by D-arginine. Cytochemical staining of the epithelial cells showed marked reactivity of NADPH diaphorase activity. These results suggest that NO is spontaneously released by the airway epithelium and that TJ-96 stimulates the epithelial NO generation.

• 社団法人 日本薬理学会の論文
• 1995-09-01

著者

• Konno Kimio

  First Department Of Medicine Tokyo Women's Medical College

• Konno Kimio

  First Department Of Internal Medicine Tokyo Women's Medical College

• Takemura Hisashi

  First Department Of Medicine Tokyo Women's Medical College

• Chiyotani Atsushi

  First Department Of Medicine Tokyo Women's Medical College

• Tamaoki Jun

  First Department Of Medicine Tokyo Women's Medical College

• Kondo Mitsuko

  First Department Of Medicine Tokyo Women's Medical College

• Tamaoki Jun

  Hiroshima University

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