Barley allele-specific amplicons useful for identifying wheat-barley recombinant chromosomes

スポンサーリンク

概要

• 論文の詳細を見る

• Barley (Hordeum vulgare L.) is potentially a new source of genes for wheat (Triticum aestivum L.) improvement. Wheat-barley chromosome recombinant lines provide a means for introgressing barley genes to wheat genome by chromosome engineering, and since these are expected to occur only rarely in special cytogenetic stocks, an efficient selection skill is necessary to identify them. To convert RFLP markers to barley allele-specific PCR markers useful for effective production of wheat-barley recombinant lines, 91 primer sets derived from RFLP clones which were previously mapped to the barley chromosomes were examined for PCR amplification using `Chinese Spring wheat, `Betzes barley and the wheat-barley chromosome addition lines. The polymorphisms were detected by an agarose gel electrophoresis of the PCR products without digestion with restriction enzymes. Out of 81 primer sets producing polymorphisms between the wheat and barley genomes, 26 amplified barley chromosome-specific DNAs which were confirmed to be located on the same chromosome as the RFLP markers by using the wheat-barley chromosome addition lines. These amplified DNAs represent barley allele-specific amplicons, which distinguish barley alleles from their wheat homoeologous counterparts. The present investigation revealed a higher probability for obtaining allele-specific amplicons from genomic DNA-derived RFLP markers than from cDNA-derived ones. The barley allele-specific amplicons developed in this study, namely, four for chromosome 2H, two for 3H, seven for 4H, eight for 5H, one for 6H and four for 7H, are suitable for identifying `Chinese Spring wheat- `Betzes barley recombinant chromosomes. However, one out of eight barley allele-specific amplicons on chromosome 5H did not detect a unique barley band in a `New Golden barley chromosome 5H addition line of `Shinchunaga wheat, indicating there may be a need to reconstruct allele-specific amplicons with different barley cultivars.

• 日本遺伝学会の論文

著者

• Taketa Shin

  Department Of Life Sciences Faculty Of Agriculture Kagawa University

• Murai Koji

  Department Of Bioscience Fukui Prefectural University

• Islam A

  Department Of Plant Science Waite Campus University Of Adelaide

• ISLAM A.K.M.Rafiqul

  Department of Plant Science, Waite Campus, University of Adelaide,

• SHEPHERD Ken

  Department of Plant Science, Waite Campus, University of Adelaide,

• Shepherd Ken

  Department Of Plant Science Waite Campus University Of Adelaide

• Islam A.

  Department Of Agronomy Waite Agricultural Research Institute University Of Adelaide

関連論文

• WFL, a wheat FLORICAULA/LEAFY ortholog, is associated with spikelet formation as lateral branch of the inflorescence meristem
• Alteration of respiration capacity and transcript accumulation level of alternative oxidase genes in necrosis lines of common wheat
• Clinical Outcome and Quality of Life of Octogenarian Patients Following Percutaneous Coronary Intervention or Surgical Coronary Revascularization
• Compensating ability in pollen fertilization between group-6 and -7 homoeologous chromosomes of barley and wheat
• Construction of a full-length cDNA library from young spikelets of hexaploid wheat and its characterization by large-scale sequencing of expressed sequence tags
• Screening of Chrysanthemum Plants with Strong Resistance to Chrysanthemum Stunt Viroid
• The einkorn wheat (Triticum monococcum) mutant, maintained vegetative phase, is caused by a deletion in the VRN1 gene
• Correlation of Cellular Life Span with Growth Parameters Observed in Successive Cultures of Human Keratinocytes
• Chrysanthemum flower shape modification by suppression of chrysanthemum-AGAMOUS gene
• Chromosomal assignment and deletion mapping of barley EST markers
• Angiomyolipoma of kidney involving the inferior vena cava
• The spatial expression patterns of DROOPING LEAF orthologs suggest a conserved function in grasses
• Genetic analysis of the STR system D20S161 in Japanese and Bangladeshi
• Distinct genetic regulation of flowering time and grain-filling period based on empirical study of D-genome diversity in synthetic hexaploid wheat lines
• orf260^, a Novel Mitochondrial Gene, is Associated with the Homeotic Transformation of Stamens into Pistil-Like Structures (Pistillody) in Alloplasmic Wheat
• Barley allele-specific amplicons useful for identifying wheat-barley recombinant chromosomes
• WAP1, a Wheat APETALA1 Homolog, Plays a Central Role in the Phase Transition from Vegetative to Reproductive Growth
• ISOLATION AND CHARACTERIZATION OF APETALA1 HOMOLOG IN WHEAT (Triticum aestivum L. )
• Expression of NCAM-H and S100 Protein in the Rostral Migratory Stream of the Adult Guinea Pig Forebrain
• Morphological basis of nitric oxide production and its correlation with the polysialylated precursor cells in the dentate gyrus of the adult guinea pig hippocampus
• Microbial Production of Biogas from Organic Wastes
• B64 ANORECTAL BLEEDING AS A SURGICAL CAUSES IN BANGLADESHI CHILDREN.
• Effect of Ppd-1 on the expression of flowering-time genes in vegetative and reproductive growth stages of wheat
• Differential effects of Aegilops tauschii genotypes on maturing-time in synthetic hexaploid wheats
• Clinical Features and Outcome in Hospitalized Heart Failure in Japan (From the ATTEND Registry)
• Efficacy and Limitations of Oral Inotropic Agents for the Treatment of Chronic Heart Failure:A Single Center Cross-Sectional Observational Study
• ClinicaE Features and Outcome in Hospitalized Heart Failure in Japan (From the ATTEND Registry)

もっと見る 閉じる

スポンサーリンク