Meiotic contraction of CAG repeats in Saccharomyces cerevisiae
スポンサーリンク
概要
- 論文の詳細を見る
Several human neurodegenerative disorders are caused by expansion of CAG repeats that occurs during meiosis or gametogenesis. We anticipated that the CAG repeats cloned in a plasmid of Saccharomyces cerevisiae might undergo a change in the number of repeats during meiosis and sporulation. To test this possibility, we devised a new method to change in vitro the number of CAG repeats and constructed plasmids carrying (CAG)39, (CAG) 65 or (CAG)123 from a plasmid carrying (CAG)18. We monitored the number of colonies showing an altered length of the repeat tracts during mitosis and meiotic growth. Contraction of long CAG repeat was found to occur frequently, whereas a few cases of expansion were observed. The contraction was equally enhanced in both orientations when the host cells grew through meiosis. Thus, our results suggest that long CAG repeats are destabilized during meiosis or gametogenesis in S. cerevisiae.
- 日本遺伝学会の論文
著者
-
Niimi Naoko
Laboratory Of Nucleic Acid Science Department Of Applied Bilogical Science College Of Bioresource Sc
-
ARAI Naoto
Laboratory of Nucleic Acid Science, Department of Applied Bilogical Science, College of Bioresource
-
AKIYAMA Ryoko
Laboratory of Nucleic Acid Science, Department of Applied Bilogical Science, College of Bioresource
-
NAKATSUBO Hiroaki
Laboratory of Nucleic Acid Science, Department of Applied Bilogical Science, College of Bioresource
-
INOUE Tadashi
Laboratory of Nucleic Acid Science, Department of Applied Bilogical Science, College of Bioresource
-
Arai Naoto
Laboratory Of Nucleic Acid Science Department Of Applied Bilogical Science College Of Bioresource Sc
-
Akiyama Ryoko
Laboratory Of Nucleic Acid Science Department Of Applied Bilogical Science College Of Bioresource Sc
-
Nakatsubo Hiroaki
Laboratory Of Nucleic Acid Science Department Of Applied Bilogical Science College Of Bioresource Sc
-
Inoue Tadashi
Laboratory Of Nucleic Acid Science Department Of Applied Bilogical Science College Of Bioresource Sc
-
INOUE Tadashi
Laboratory of Mutagenesis, National Institute of Genetics
関連論文
- Meiotic contraction of CAG repeats in Saccharomyces cerevisiae
- サルモネラおよび枯草菌の自然突然変異に対するL-エチオニンの抗変異効果〔英文〕