Changes in Haemolymph Protease Inhibitors during Metamorphosis of the Silkworm, Bombyx mori L. (Lepidoptera : Bombycidae)

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Changes in the activity, elution profile on a column and electrophoretic pattern of haemolymph protease inhibitors from the silkworm, Bombyx mori, were studied during larval-pupal and pupal-adult development. Inhibitory activity increased with development in the 5th larval stage, reached a peak at the onset of spinning and then decreased gradually. The difference between the highest and lowest points was 54.1% in the female and 72.3% in the male. An appreciable difference was observed in the elution patterns of the inhibitor on a Sephadex G-75 column in the successive stages of development. By agar gel electrophoresis, at least three inhibitor bands were resolved for trypsin and for α-chymotrypsin respectively. The inhibitor bands against these proteases did not correspond to each other except for a negatively moving band. However, there was a correspondence between the transition in intensity of inhibitor bands and the change in inhibitor activity during development. In view of these results, the possible significance of protease inhibitors in silkworm haemolymph was discussed.
日本応用動物昆虫学会の論文
1982-05-25