Restriction fragment length polymorphism analysis of Haemophilus influenzae type b strains isolated simultaneously from cerebrospinal fluid, blood, and nasopharynx of Japanese children with bacterial meningitis
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概要
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During the 10 years preceding the introduction of the Haemophilus influenzae type b (Hib) vaccine, restriction fragment length polymorphism (RFLP) analysis using pulsed-field gel electrophoresis was performed on 66 strains (22 sets) of Hib isolated simultaneously from cerebrospinal fluid, blood, and nasopharyngeal samples of 22 patients with meningitis. Strains from the 3 types of samples showed identical RFLP patterns in 18 of the 22 patients, confirming the pathway of Hib infection starting from the nasopharynx and leading to meningitis through bacteremia. In the remaining 4 patients, the RFLP patterns from the 3 sample types were also nearly identical. However, a single band in the RFLP patterns of strains from 1 of the 3 sample types showed a shift of about 20 kb. One copy of the cap-b gene had a molecular weight of 18 kb, and the discrepancy in the band's molecular weight indicated an amplification or loss of a single copy of the cap. These findings suggest that Hib caused meningitis by changing the number of copies of the cap to evade the host's immunological response.
著者
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石和田 稔彦
Department Of Pediatrics Graduate School Of Medicine Chiba University
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Kohno Yoichi
Department Of Pediatrics School Of Medicine Chiba University
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Ishiwada Naruhiko
Department Of Pediatrics Graduate School Of Medicine Chiba University
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石和田 稔彦
Department Of Pediatrics Chiba University Graduate School Of Medicine
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Hoshino Tadashi
Division Of Infectious Disease Chiba Children's Hospital
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Kohno Yoichi
Department Of Pediatrics Chiba University Hospital
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