東日本地域の水道水と一級河川水の細胞毒性

概要

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Water was collected from the taps of houses in cities and from first-class rivers in eastern Japan, and organic compounds in the water were extracted and concentrated using an Oasis HLB 3-cc extraction cartridge. Human RSa cells were examined for assessing the cytotoxic effects of the concentrated compounds present in water by MTT assay. The viability of cells treated with tap water samples was found to be about 80-100Pompared with 100 168822344n cells treated with MilliQ water samples. The viability of cells treated with 17 river water samples was over 80 3.251414e+161xcept for the sample from the Aganogawa river; water samples from Tamagawa and Arakawa rivers showed less than 70% viability in their middle and lower sections. The deterioration in water quality was particularly evident in April and July in Tamagawa river and in August and October in Edogawa river. Tests of the cytotoxic effects of water samples using human RSa cells may be a more comprehensive approach in evaluating biological effects of various factors in tap and river water and in unifying the criteria that apply to environmental water quality.