青枯病抵抗性選抜トマト系統の果実特性試験と養液栽培における青枯病抵抗性検定法の確立

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[Synopsis] In this study, fruit qualities of the bacterial wilt-resistant tomato line (LNSR-7) were evaluated in order to breed a new commercial cultivar. Additionally, the method for inoculating the bacterial wilt pathogen into tomato plants was examined using a hydroponic culture system. First, self-pollinated plants (R0-R8) of the resistant line were cultivated in the field and examined for their growth, plant appearance, and fruit productivities and qualities. All of tested tomato lines grew similarly to those of commercial cultivars used as control, and some progenies were found superior in plant appearance, fruit shape, and brix than the control cultivars. In the present study, four progenies (R6-37, R7-05, 21 and R8-23) were finally selected as resistant tomato plants with superior commercial characteristics. In the second experiment, the hydroponic culture method was adopted for achieving an effective inoculation of the pathogenic bacterium Ralstonia solanacearum (Pseudomonas solanacearum) . The density (10^6-10^8 cells/ml) of the pathogen inoculated into a hydroponic culture solution was stably maintained during the entire periods of experiment and was shown to be enough to cause the wilting in all of tested tomato plants within 9 days after inoculation. Thus, the present study revealed an effective isolation and utilization of useful somaclonal variations for producing bacterial wilt-resistant tomato plants with high commercial characteristics, and established an effective inoculation method of the bacterial wilt pathogen for rapidly evaluating the resistance of selected tomato plants.
近畿大学農学総合研究所の論文

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備後美紀
近畿大農

青枯病抵抗性選抜トマト系統の果実特性試験と養液栽培における青枯病抵抗性検定法の確立

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